Evidence map›Paper›PMID 35608299›Full record

ArticlemBio2022

Shintaro Hara, Naohisa Wada, Sliver Sung-Yun Hsiao, Meng Zhang, Zhihua Bao, Yoshiyuki Iizuka, Der-Chuen Lee, Shusei Sato, Sen-Lin Tang, Kiwamu Minamisawa

Open access · goldAbstract read
In one paragraph

Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Variations in the NRice (New York, N.Y.) · 2025
    Article
  6. Article
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  8. Article
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  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Shintaro Hara *Graduate School of Life Sciences, Tohoku University, Sendai, Japan.ORCID 0000-0003-1429-5551
Naohisa Wada *Biodiversity Research Center, Academia Sinicagrid.28665.3f, Taipei, Taiwan.ORCID 0000-0001-5949-9115
Sliver Sung-Yun HsiaoInstitute of Astronomy and Astrophysics, Academia Sinicagrid.28665.3f, Taipei, Taiwan.ORCID 0000-0002-9774-369X
Meng ZhangInner Mongolia Key Laboratory of Environmental Pollution Prevention and Resource Recycle, Hohhot, China.ORCID 0000-0003-1557-101X
Zhihua BaoInner Mongolia Key Laboratory of Environmental Pollution Prevention and Resource Recycle, Hohhot, China.ORCID 0000-0002-2741-484X
Yoshiyuki IizukaInstitute of Earth Science, Academia Sinicagrid.28665.3f, Taipei, Taiwan.ORCID 0000-0002-7327-7583
Der-Chuen LeeInstitute of Earth Science, Academia Sinicagrid.28665.3f, Taipei, Taiwan.ORCID 0000-0001-8855-9594
Shusei SatoGraduate School of Life Sciences, Tohoku University, Sendai, Japan.ORCID 0000-0002-0293-5366
Sen-Lin TangBiodiversity Research Center, Academia Sinicagrid.28665.3f, Taipei, Taiwan.ORCID 0000-0002-5852-972X
Kiwamu MinamisawaGraduate School of Life Sciences, Tohoku University, Sendai, Japan.ORCID 0000-0001-7302-4079
Biodiversity Research Center, Academia Sinica · TWInstitute of Earth Sciences, Academia Sinica · TWTohoku University · JPInstitute of Astronomy and Astrophysics, Academia Sinica · TWNational Agriculture and Food Research Organization · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methane-oxidizing bacteria (methanotrophs) play an ecological role in methane and nitrogen fluxes because they are capable of nitrogen fixation and methane oxidation, as indicated by genomic and cultivation-dependent studies. However, the chemical relationships between methanotrophy and diazotrophy and aerobic and anaerobic reactions, respectively, in methanotrophs remain unclear. No study has demonstrated the cooccurrence of both bioactivities in a single methanotroph bacterium in its natural environment. Here, we demonstrate that both bioactivities in type II methanotrophs occur at the single-cell level in the root tissues of paddy rice (Oryza sativa L. cv. Nipponbare). We first verified that difluoromethane, an inhibitor of methane monooxygenase, affected methane oxidation in rice roots. The results indicated that methane assimilation in the roots mostly occurred due to oxygen-dependent processes. Moreover, the results indicated that methane oxidation-dependent and methane oxidation-independent nitrogen fixation concurrently occurred in bulk root tissues. Subsequently, we performed fluorescence

Indexed as

OryzaIn Situ Hybridization, FluorescenceIsotopesMethaneNitrogenOxidation-ReductionSoil MicrobiologyIsotopesMethaneNitrogendiazotrophymethanotrophyNanoSIMSpaddy ricesingle celltype II methanotrophs

Identifiers

PMID35608299
PMCPMC9239180
OpenAlexW4282916027

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.