ArticleDevelopment (Cambridge, England)2022
Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Cross-species comparative transcriptomic analysis of spermatozoa reveals species-specific regulatory networks linked to fertilization-associated genes in cattle, sheep and goats.Science China. Life sciences · 2026Article
- XPF-like domain in human SHOC1 is required for crossover formation and protecting autosome from MSUC.Nucleic acids research · 2026Article
- Digital Chromosome Banding Reveals Distinct Spatiotemporal Dynamics and Sexual Dimorphism in Meiotic Silencing.bioRxiv : the preprint server for biology · 2026Article
- SARS-CoV-2 infection reduces the number of spermatogonial stem cells and dysregulates the transcriptional landscape of the human testis.Research square · 2026Article
- Meiotic prophase I disruption as a strategy for nonhormonal male contraception using small-molecule inhibitor JQ1.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Fertilization with Protamine-2 deficient sperm triggers abnormal pronucleus development and zygotic cleavage†.Biology of reproduction · 2026Article
- Stability of epigenetic transgenerational inheritance of adult-onset disease and parturition abnormalities.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Dynamic architecture of mammalian paternal chromatin: histone-to-protamine exchange and post-fertilization reprogramming.Epigenetics & chromatin · 2025Review
- A father's legacy: the sperm epigenome, preimplantation development, and paternal environment.Epigenomics · 2025Review
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Palmitoyltransferase ZDHHC19 regulates histone-to-protamine exchange during spermiogenesis in mice.Science advances · 2025Article
- Protamine expression in somatic cells condenses chromatin and disrupts transcription without altering DNA methylation.Epigenetics & chromatin · 2025Article
- EP300-interacting inhibitor of differentiation 3 is required for spermatogenesis in mice.Andrology · 2025Article
- Germline stem cell isolation, lineage tracing, and aging in a protochordate.bioRxiv : the preprint server for biology · 2025Article
- Article
- Male germ cells with Bag5 deficiency show reduced spermiogenesis and exchange of basic nuclear proteins.Cellular and molecular life sciences : CMLS · 2025Article
- Thymoquinone-Loaded Chitosan Nanoparticles Combat Testicular Aging and Oxidative Stress Through SIRT1/FOXO3a Activation: An In Vivo and In Vitro Study.Pharmaceutics · 2025Article
- Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis.Nucleic acids research · 2025Article
- Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis.Nucleic acids research · 2025Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the key events during spermiogenesis is the hypercondensation of chromatin by substitution of the majority of histones by protamines. In humans and mice, protamine 1 (PRM1/Prm1) and protamine 2 (PRM2/Prm2) are expressed in a species-specific ratio. Using CRISPR-Cas9-mediated gene editing, we generated Prm1-deficient mice and demonstrated that Prm1+/- mice were subfertile, whereas Prm1-/- mice were infertile. Prm1-/- and Prm2-/- sperm showed high levels of reactive oxygen species-mediated DNA damage and increased histone retention. In contrast, Prm1+/- sperm displayed only moderate DNA damage. The majority of Prm1+/- sperm were CMA3 positive, indicating protamine-deficient chromatin, although this was not the result of increased histone retention in Prm1+/- sperm. However, sperm from Prm1+/- and Prm1-/- mice contained high levels of incompletely processed PRM2. Furthermore, the PRM1:PRM2 ratio was skewed from 1:2 in wild type to 1:5 in Prm1+/- animals. Our results reveal that PRM1 is required for proper PRM2 processing to produce mature PRM2, which, together with PRM1, is able to hypercondense DNA. Thus, the species-specific PRM1:PRM2 ratio has to be precisely controlled in order to retain full fertility.
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