Evidence map›Paper›PMID 35607416›Full record

ArticleMaterials today. Bio2022

Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes.

Chenwei Xiong, Jie Li, Luyao Li, Long Chen, Rong Zhang, Xianqiang Mi, Yifan Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Materials today. Bio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Chenwei XiongDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Jie LiDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Luyao LiDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Long ChenDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Rong ZhangDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Xianqiang MiShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.
Yifan LiuDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
ShanghaiTech University · CNChinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, features high cost-effectiveness and convenient fabrication. This is realized by patterning a micrometer-sized heterojunction consisting of adjacent segments of polycationic and polyanionic hydrogels on a microfluidic chip through simple photocuring steps. The integrated diode rectifies ionic currents being sensitive to the charge of DNA adsorbed onto the polycationic chains through electrostatic associations. Based on the mechanism, we show that the ionic biosensor can electrically quantify DNA in a dynamic range relevant to typical nucleic acid amplification assays. Utilizing the device, we demonstrate the evaluation of a PCR assay amplifying a 500-bp DNA fragment of

Indexed as

Electrical biosensorHydrogelIonic diodeNucleic acidPoint-of-care

Identifiers

PMID35607416
PMCPMC9123263
OpenAlexW4280532773

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.