ArticleAMB Express2022
Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea.
Article in AMB Express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Ectomycorrhizal fungi increase phosphorus uptake in Pinus sylvestris var. mongolica and the mechanism controlling Sphaeropsis sapinea.BMC plant biology · 2026Article
- Biocontrol Effects and Potential Defence Mechanism of Bacillus velezensis JS6-1 Against Fusarium solani-Induced Postharvest Rots in Muskmelon.Current microbiology · 2025Article
- Optimization of Fermentation and Biocontrol Efficacy ofMicroorganisms · 2024Article
- Optimization of the Fermentation Conditions ofMicroorganisms · 2023Article
- Article
- Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites fromJournal of fungi (Basel, Switzerland) · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
A previous study found that a biocontrol bacterium, Bacillus pumilus HR10, inhibited the Sphaeropsis shoot blight disease of pine, and the fermentation broth of HR10 strain contained protein antifungal substances. The optimal formulation of the fermentation medium for the antagonistic substance of B. pumilus HR10 was finally obtained by single-factor test, Packett-Burman test, steepest ascent test and Box-Behnken Design (BBD) response surface test, and the best formulation of the fermentation medium for the antagonistic substance of B. pumilus HR10 was 12 g/L corn meal, 15 g/L beef extract and 13 g/L magnesium sulfate, with a predicted bacterial inhibition rate of 89%. The fermentation filtrate of B. pumilus HR10 cultured with the optimized medium formulation was verified to have an inhibition rate of (87.04 ± 3.2) % on the growth of Sphaeropsis sapinea by three replicate tests. The antagonistic crude protein of B. pumilus HR10 were further isolated and identified using HiTrap Capto Q strong Ion-Exchange Chromatography and LC-MS-MS, and it was speculated that glycoside hydrolase (Ghy), beta-glucanase (Beta), arabinogalactan endonuclease β-1,4-galactanase (Arab), and immunosuppressant A (ImA) are proteins with antagonistic activity against S. sapinea in the B. pumilus HR10.
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