ArticleNature communications2022
Complex dynamics in a synchronized cell-free genetic clock.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Deep learning for predicting the occurrence of tipping points.Royal Society open science · 2025Article
- From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator.Nature communications · 2024Article
- Learning metabolic dynamics from irregular observations by Bidirectional Time-Series State Transfer Network.mSystems · 2024Article
- Cell-Free Gene Expression in Bioprinted Fluidic Networks.ACS synthetic biology · 2024Article
- Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A general model-based causal inference method overcomes the curse of synchrony and indirect effect.Nature communications · 2023Article
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Authors and funding
3 authors.
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Abstract
Complex dynamics such as period doubling and chaos occur in a wide variety of non-linear dynamical systems. In the context of biological circadian clocks, such phenomena have been previously found in computational models, but their experimental study in biological systems has been challenging. Here, we present experimental evidence of period doubling in a forced cell-free genetic oscillator operated in a microfluidic reactor, where the system is periodically perturbed by modulating the concentration of one of the oscillator components. When the external driving matches the intrinsic period, we experimentally find period doubling and quadrupling in the oscillator dynamics. Our results closely match the predictions of a theoretical model, which also suggests conditions under which our system would display chaotic dynamics. We show that detuning of the external and intrinsic period leads to more stable entrainment, suggesting a simple design principle for synchronized synthetic and natural genetic clocks.
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