ArticlePlant communications2022
Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses.
Article in Plant communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed, 79 citations in OpenAlex.
- Multi-organ single-cell transcriptomic atlas identifies QrIAA14 as a candidate negative regulator of adventitious root development in Quercus robur.PLoS genetics · 2026Article
- Longitudinal zone- and cell type-specific pattern-triggered immune responses in Arabidopsis roots.Developmental cell · 2026Article
- Resetting of epigenetic cold memory through somatic embryogenesis in plant regeneration.Nature plants · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- PLETHORA-autophagy axis activates organ regeneration through ROS modulation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The role of jasmonates in plant root growth and development.Frontiers in plant science · 2026Review
- Single-cell transcriptomics reveals cellular and genetic mechanisms of alpine adaptation inFrontiers in plant science · 2026Article
- Influence ofJournal of fungi (Basel, Switzerland) · 2025Article
- Article
- Proline transporters balance the salicylic acid-mediated trade-off between regeneration and immunity in plants.bioRxiv : the preprint server for biology · 2025Article
- Single-cell transcriptome atlas unveils transcriptional regulation networks of banana root tips in response toHorticulture research · 2025Article
- Hormone functions in adventitious root formation during cutting propagation of woody plants.Journal of plant research · 2025Review
- Single-cell views of fate reprogramming in de novo organogenesis.Journal of plant research · 2025Review
- snRNA-Seq Unveils Cell-Type-Specific Immune Dynamics in Arabidopsis During Pinewood Nematode Infection.Molecular plant pathology · 2025Article
- Single-cell omics in plant biology: mechanistic insights and applications for crop improvement.Advanced biotechnology · 2025Review
- Water availability positions auxin response maxima to determine plant regeneration fates.Nature plants · 2025Article
- RALF33-FERONIA signaling orchestrates postwounding root-tip regeneration via TPR4-ERF115 dynamics.The Plant cell · 2025Article
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Regeneration and defense: unveiling the molecular interplay in plants.The New phytologist · 2025Review
- Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses.Quantitative biology (Beijing, China) · 2025Review
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detached Arabidopsis thaliana leaves can regenerate adventitious roots, providing a platform for studying de novo root regeneration (DNRR). However, the comprehensive transcriptional framework of DNRR remains elusive. Here, we provide a high-resolution landscape of transcriptome reprogramming from wound response to root organogenesis in DNRR and show key factors involved in DNRR. Time-lapse RNA sequencing (RNA-seq) of the entire leaf within 12 h of leaf detachment revealed rapid activation of jasmonate, ethylene, and reactive oxygen species (ROS) pathways in response to wounding. Genetic analyses confirmed that ethylene and ROS may serve as wound signals to promote DNRR. Next, time-lapse RNA-seq within 5 d of leaf detachment revealed the activation of genes involved in organogenesis, wound-induced regeneration, and resource allocation in the wounded region of detached leaves during adventitious rooting. Genetic studies showed that BLADE-ON-PETIOLE1/2, which control aboveground organs, PLETHORA3/5/7, which control root organogenesis, and ETHYLENE RESPONSE FACTOR115, which controls wound-induced regeneration, are involved in DNRR. Furthermore, single-cell RNA-seq data revealed gene expression patterns in the wounded region of detached leaves during adventitious rooting. Overall, our study not only provides transcriptome tools but also reveals key factors involved in DNRR from detached Arabidopsis leaves.
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