Evidence map›Paper›PMID 35600930›Full record

ArticleSTAR protocols2022

Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics.

Thomas T Schulze, Andrew J Neville, Ryan C Chapman, Paul H Davis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Thomas T SchulzeDepartment of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
Andrew J NevilleDepartment of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
Ryan C ChapmanDepartment of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
Paul H DavisDepartment of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
University of Nebraska at Omaha · US

Funding

UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI PAUL L SORGEN · 2012 to 2026
$59.2M
UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Joann B. Sweasy · 1985 to 2026
$55.0M
Optimization of Antischistosomal ChemotypesR01AI116723 · NIAID · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI VENNERSTROM, JONATHAN L · 2015 to 2024
$6.2M
NCI NIH HHS P30 CA036727NIAID NIH HHS R01 AI116723NIGMS NIH HHS P20 GM103427
6 · The paper itself

Abstract

Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape.

Indexed as

SpleenTranscriptomeAnimalsFlow CytometryMammalsMiceSingle-Cell AnalysisAntibodyBioinformaticsCell BiologyCell isolationCell separation/fractionationFlow Cytometry/Mass CytometryGene ExpressionImmunologyModel OrganismsMolecular BiologyRNAseqSequence analysisSequencingSingle Cell

Identifiers

PMID35600930
PMCPMC9120244
OpenAlexW4280554926

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.