Evidence map›Paper›PMID 35600925›Full record

ArticleSTAR protocols2022

Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates.

Haneul Yoo, D Allan Drummond

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Haneul YooDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
D Allan DrummondDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.

Funding

Molecular And Cellular Biology Training ProgramT32GM007183 · NIGMS · UNIVERSITY OF CHICAGO · PI GLICK, BENJAMIN S, RICE, PHOEBE A · 1985 to 2021
$23.9M
Function and Regulation of Stress-Induced Adaptive CondensatesR35GM144278 · NIGMS · UNIVERSITY OF CHICAGO · PI David Allan Drummond · 2022 to 2026
$2.6M
Determining the molecular basis of adaptive stress-triggered protein phase separationR01GM126547 · NIGMS · UNIVERSITY OF CHICAGO · PI DRUMMOND, DAVID ALLAN, SOSNICK, TOBIN R · 2018 to 2021
$1.6M
Signatures of interaction-driven selection on low-complexity sequencesR01GM127406 · NIGMS · UNIVERSITY OF CHICAGO · PI DRUMMOND, DAVID ALLAN · 2018 to 2021
$1.1M
Investigating the disaggregation of stress-induced phase-separated poly(A)-binding protein (Pab1) by its cognate disaggregation systemF31ES030697 · NIEHS · UNIVERSITY OF CHICAGO · PI YOO, HANEUL · 2019 to 2020
$91k
NIEHS NIH HHS F31 ES030697NIGMS NIH HHS R01 GM126547NIGMS NIH HHS R01 GM127406NIGMS NIH HHS R35 GM144278NIGMS NIH HHS T32 GM007183
6 · The paper itself

Abstract

Heat stress triggers a specific set of proteins in budding yeast to form solid-like biomolecular condensates, which are dispersed by molecular chaperones. Here, we describe a protocol to study the kinetics of chaperone-facilitated condensate dispersal using biochemical reconstitution and fluorescence anisotropy. Although the current protocol is tailored to study heat-induced condensates of poly(A)-binding protein (Pab1), the protocol can be modified to study any protein which shows differential substrate binding activity upon condensation. For complete details on the use and execution of this protocol, please refer to Yoo et al. (2022).

Indexed as

Molecular ChaperonesRNA-Binding ProteinsFluorescence PolarizationHeat-Shock ResponsePoly(A)-Binding ProteinsMolecular ChaperonesPoly(A)-Binding ProteinsRNA-Binding ProteinsBiophysicsCell BiologyMolecular BiologyProtein Biochemistry

Identifiers

PMID35600925
PMCPMC9121323

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.