Evidence map›Paper›PMID 35600892›Full record

ArticleFrontiers in bioengineering and biotechnology2022

FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in

Jesse A Coker, Vittorio L Katis, Michael Fairhead, Anja Schwenzer, Stine B Clemmensen, Bent U Frandsen, Willem A de Jongh, Opher Gileadi, Nicola A Burgess-Brown, Brian D Marsden and 2 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Jesse A CokerCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Vittorio L KatisCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Michael FairheadCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Anja SchwenzerKennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
Stine B ClemmensenExpreS2ion Biotechnologies, SCION-DTU Science Park, Hørsholm, Denmark.
Bent U FrandsenExpreS2ion Biotechnologies, SCION-DTU Science Park, Hørsholm, Denmark.
Willem A de JonghExpreS2ion Biotechnologies, SCION-DTU Science Park, Hørsholm, Denmark.
Opher GileadiCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Nicola A Burgess-BrownCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Brian D MarsdenCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Kim S MidwoodKennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
Wyatt W YueCentre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
University of Oxford · GBExpreS2ion Biotechnologies (Denmark) · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the

Indexed as

glycoproteinsinsect cellsrecombinant proteinsS2 cellssecreted proteinsstructural biology

Identifiers

PMID35600892
PMCPMC9117644
OpenAlexW4229032942

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.