Evidence map›Paper›PMID 35599106›Full record

ArticleJournal of advanced research2023

New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress.

Xu Chen, Jinhong Wu, Fujia Yang, Mi Zhou, Ruibin Wang, Jianlian Huang, Yuzhi Rong, Jianhua Liu, Shaoyun Wang

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 19 citations in OpenAlex.

  1. Molecular Mechanisms of Basil (Molecules (Basel, Switzerland) · 2026
    Article
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  3. FFoods (Basel, Switzerland) · 2026
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Xu ChenSchool of Mechanical Science & Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
Jinhong WuDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Fujia YangCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
Mi ZhouDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Ruibin WangInstrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China.
Jianlian HuangKey Laboratory of Refrigeration and Conditioning Aquatic Products Processing of Ministry of Agriculture and Rural Affairs, Xiamen 361022, China.
Yuzhi RongSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China.
Jianhua LiuInternational Faculty of Applied Technology, Yibin University, Yibin, Sichuan 644000, China.
Shaoyun WangCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: shywang@fzu.edu.cn.
Fuzhou University · CNShanghai Jiao Tong University · CNMinistry of Agriculture and Rural Affairs · CNShanghai Institute of Technology · CNYibin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionAntifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in this process.

objectivesOur study aims to investigate how antifreeze peptides regulate cell physiological functions during the freezing process.

methodsWe investigated the cryoprotective effect of rsfAFP on the physiological functions of S. thermophilus under freezing stress by measuring cellular metabolism activity, intracellular enzyme activity, cell membrane characterization, and cell apoptosis. The mechanism by which rsfAFP impacts S. thermophilus physiological functions under freezing stress was investigated using multispectral techniques and cryo-TEM.

resultsWe show that a recombinant antifreeze peptide (rsfAFP) interacts with the extracellular capsular polysaccharides and peptidoglycan of Streptococcus thermophilus and ice to cover the outer layer of the membrane, forming a dense protective layer that regulates the molecular structure of extracellular ice crystals, which results in reduced extracellular membrane damage, depressed apoptosis and increased intracellular metabolic activity. This interaction mechanism was indicated by the fact that S. thermophilus better maintained its permeability barrier, membrane fluidity, membrane structural integrity, and cytoplasmic membrane potential during freezing stress with rsfAFP treatment.

conclusionThese results provide new insights into the mechanism by which rsfAFP regulates frozen cellphysiological functionsand apoptosis under freezing stress.

Indexed as

IceStreptococcus thermophilusAntifreeze ProteinsCryoprotective AgentsFreezingAntifreeze ProteinsCryoprotective AgentsIceAntifreeze peptideCryoprotective mechanismMembrane structurePhysiological functionStreptococcus thermophilus

Identifiers

PMID35599106
PMCPMC10006524
OpenAlexW4229441842

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.