ArticleScientific reports2022
Comparative analysis of mesenchymal stem cells cultivated in serum free media.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Evaluation of the Expression and Prognosis of Neural-Related Products Expressed by Cancer-Derived Mesenchymal Stem Cells With the Characteristics of Neural Stem Cells.Cancer medicine · 2026Article
- The Secretome Derived From Serum-Free Media of SHED-MSCs Can Modulate THP-1-Derived Macrophage Cells.Journal of cellular and molecular medicine · 2026Article
- Basic fibroblast growth factor preserves rabbit adipose-derived mesenchymal stromal cells in serum-free culture.Journal of veterinary science · 2026Article
- Validation of a novel genomic biomarker of mesenchymal stem cell scalability and implications of genotype status on cellular senescence phenotypes.Scientific reports · 2026Article
- Establishment of human-relevantFrontiers in toxicology · 2026Article
- Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins.Regenerative therapy · 2025Article
- Conditioning period impacts the morphology and proliferative effect of extracellular vesicles derived from rat adipose tissue derived stromal cell.Journal of nanobiotechnology · 2025Article
- Optimization of Culture Media for Human Umbilical Cord-Derived Mesenchymal Stem Cell Production.Stem cells international · 2025Article
- Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media.Frontiers in toxicology · 2025Review
- Current advancements in nanotechnology for stem cells.International journal of surgery (London, England) · 2024Review
- Comparative Analysis of Serum and Serum-Free Medium Cultured Mesenchymal Stromal Cells for Cartilage Repair.International journal of molecular sciences · 2024Article
- Starvation and Inflammation Modulate Adipose Mesenchymal Stromal Cells' Molecular Signature.Journal of personalized medicine · 2024Article
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation.Journal of functional biomaterials · 2024Review
- Comprehensive assessment of goat adipose tissue-derived mesenchymal stem cells cultured in different media.Scientific reports · 2024Article
- Development of Good Manufacturing Practice-Compatible Isolation and Culture Methods for Human Olfactory Mucosa-Derived Mesenchymal Stromal Cells.International journal of molecular sciences · 2024Article
- Assessment of the Dose-Dependent Effect of Human Platelet Lysate on Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells Culture for Manufacturing Protocols.Stem cells and cloning : advances and applications · 2024Article
- Article
- Spheroid trilineage differentiation model of primary mesenchymal stem/stromal cells under hypoxia and serum-free culture conditions.Frontiers in bioengineering and biotechnology · 2024Article
- Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells.Stem cell research & therapy · 2023Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.
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