ArticleScience China. Life sciences2022
Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression.
Article in Science China. Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Development of a BM7G(TKO/hCD46/hCD55/hTHBD/hEPCR) donor pig with endogenous promoter-driven transgenes for xenotransplantation.Frontiers in immunology · 2026Article
- Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy.Science China. Life sciences · 2024Article
- In vivo evaluation of guide-free Cas9-induced safety risks in a pig model.Signal transduction and targeted therapy · 2024Article
- Crosstalk between trace elements and T-cell immunity during early-life health in pigs.Science China. Life sciences · 2023Review
- Mechanism of iron on the intestinal epithelium development in suckling piglets.Science China. Life sciences · 2023Article
- Trace metal elements: a bridge between host and intestinal microorganisms.Science China. Life sciences · 2023Review
- Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation.Genome biology · 2023Article
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26 authors.
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Abstract
Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process. Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline. Previous pig models with tetracycline regulatory elements were generated through random integration. This process often resulted in uncertain expression and unpredictable phenotypes, thus hindering their applications. Here, by precise knock-in of binary Tet-On 3G elements into Rosa26 and Hipp11 locus, respectively, a double knock-in reporter pig model was generated. We characterized excellent properties of this system for controllable transgenic expression both in vitro and in vivo. Two attP sites were arranged to flank the tdTomato to switch reporter gene. Single or multiple gene replacement was efficiently and faithfully achieved in fetal fibroblasts and nuclear transfer embryos. To display the flexible application of this system, we generated a pig strain with Dox-inducing hKRAS
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