Evidence map›Paper›PMID 35596200›Full record

ArticleBMC biomedical engineering2022

Gold-based nanoplatform for a rapid lateral flow immunochromatographic test assay for gluten detection.

Arefe Momeni, Mohammad Rostami-Nejad, Reza Salarian, Mohammad Rabiee, Elham Aghamohammadi, Mohammad Reza Zali, Navid Rabiee, Franklin R Tay, Pooyan Makvandi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biomedical engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 4 countries.

Arefe MomeniBiomaterials Group, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Mohammad Rostami-NejadGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, 1985714711, Iran. m.rostamii@gmail.com.
Reza SalarianBiomedical Engineering Department, Maziar University, Royan, Iran. r.salarian@maziar.ac.ir.
Mohammad RabieeBiomaterials Group, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran. mrabiee@aut.ac.ir.
Elham AghamohammadiBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Reza ZaliBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Navid RabieeSchool of Engineering, Macquarie University, Sydney, New South Wales, 2109, Australia. nrabiee94@gmail.com.
Franklin R TayThe Graduate School, Augusta University, Augusta, GA, 30912, USA.
Pooyan MakvandiIstituto Italiano di Tecnologia, Centre for Materials Interfaces, viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy.
Shahid Beheshti University of Medical Sciences · IRAmirkabir University of Technology · IRMacquarie University · AUPiaggio Aerospace (Italy) · ITThe Graduate Center, CUNY · USUniversity of Mazandaran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGluten, a food allergen, is available in foods derived from wheat, rye and barley. It damages the small intestine and causes celiac disease. Herein, we designed a rapid immunochromatographic lateral flow test assay for detecting the gluten contents of raw materials. In this rapid test, the presence of gluten was screened through the capturing of gliadin (a toxic component of gluten) by two identical gliadin monoclonal antibodies. One of the antibodies was immobilized on the membrane in the test zone as a capture reagent. The other antibody was labeled with gold nanoparticles (AuNPs) as a detector reagent.

resultsGold nanoparticles with a size of about 20 nm were synthesized and conjugated to the gliadin monoclonal antibodies. The detection limit of the experimental assay was 20 ppm and positive results were visualized after 15 min using only 40 μL of the extracted sample for each test. Analysis of different flour samples identified the best sensitivity and specificity of the lateral flow test strip (LFTS).

conclusionThe experimental LFTS is an easy-to-use and rapid method for the screening of gluten level in raw materials. The LFTS may be employed to ensure the safety of foods.

Indexed as

AuNPsfood allergenglutenLFTSrapid testraw materials

Identifiers

PMID35596200
PMCPMC9121606
OpenAlexW4280643684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.