Evidence map›Paper›PMID 35596077›Full record

ArticleNature plants2022

Boosting plant genome editing with a versatile CRISPR-Combo system.

Changtian Pan, Gen Li, Aimee A Malzahn, Yanhao Cheng, Benjamin Leyson, Simon Sretenovic, Filiz Gurel, Gary D Coleman, Yiping Qi

Abstract read
PubMed Publisher
In one paragraph

Article in Nature plants, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
13.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed, 154 citations in OpenAlex.

  1. Review
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  5. Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026
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25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Changtian PanDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.ORCID http://orcid.org/0000-0002-5852-059X
Gen LiDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Aimee A MalzahnDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Yanhao ChengDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Benjamin LeysonDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Simon SretenovicDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.ORCID http://orcid.org/0000-0002-3080-5104
Filiz GurelDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Gary D ColemanDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Yiping QiDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA. yiping@umd.edu.ORCID http://orcid.org/0000-0002-9556-6706
University of Maryland, College Park · USUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided genome engineering in plants. However, these systems are mostly used separately, leaving their combinational potential largely untapped. Here we develop a versatile CRISPR-Combo platform, based on a single Cas9 protein, for simultaneous genome editing (targeted mutagenesis or base editing) and gene activation in plants. We showcase the powerful applications of CRISPR-Combo for boosting plant genome editing. First, CRISPR-Combo is used to shorten the plant life cycle and reduce the efforts in screening transgene-free genome-edited plants by activation of a florigen gene in Arabidopsis. Next, we demonstrate accelerated regeneration and propagation of genome-edited plants by activation of morphogenic genes in poplar. Furthermore, we apply CRISPR-Combo to achieve rice regeneration without exogenous plant hormones, which is established as a new method to predominately enrich heritable targeted mutations. In conclusion, CRISPR-Combo is a versatile genome engineering tool with promising applications in crop breeding.

Indexed as

ArabidopsisGene EditingCRISPR-Cas SystemsGenome, PlantPlant BreedingPlants, Genetically Modified

Identifiers

PMID35596077
OpenAlexW4280518325

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.