ArticleFrontiers in plant science2022
Overhauling the Effect of Surface Sterilization on Analysis of Endophytes in Tea Plants.
Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Unlocking the hidden potential of the genus Salvia through a systematic review of endophytic bacterial and fungal diversity and biotechnological prospects.Archives of microbiology · 2026Pooled it
- Endophytic Fungal Metabolites as Modulators of Key Signaling Pathways in Chronic Diseases and Aging.Antibiotics (Basel, Switzerland) · 2026Review
- From Traditional to Omics-Driven: Emerging Strategies for Isolation, Cultivation, and Identification of Plant Endophytes.Plants (Basel, Switzerland) · 2026Review
- Fungi Associated With Freshwater Zooplankton Are Taxon-Specific, Temporally Dynamic and Reflect Allochthonous Inputs.Molecular ecology · 2026Article
- Phytochemical Profile and Antioxidant Potential ofAntioxidants (Basel, Switzerland) · 2026Article
- Beauveria bassiana acts as a beneficial endophyte in tea crops, modulating microbial communities and metabolic pathways to enhance plant growth.BMC plant biology · 2026Article
- Comparative profiling of surface-sterilized leaf-associated microbiota and flavor-associated metabolites in two Gougunao tea cultivars across fresh and fermented leaf stages.Frontiers in nutrition · 2026Article
- Article
- qPCR-based quantification reveals high plant host-specificity of endophytic colonization levels in leaves.American journal of botany · 2025Article
- Wild again: recovery of a beneficial Cannabis seed endophyte from low domestication genotypes.Microbiome · 2024Article
- The Populations of Two Differently Medicine-Used Plants of Hedyotis diffusa and Hedyotis corymbosa Shoot-Assembling Rich Bacterial and Fungal Communities with Varied Compositions but Conserved Structures.Current microbiology · 2024Article
- Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- Analyzing the defense response mechanism ofFrontiers in plant science · 2024Article
- In Planta Detection ofPlants (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing evidence shows that plant Endophytes play a crucial role in the fitness and productivity of hosts. Surface sterilization is an indispensable process before high-throughput sequencing (HTS) and tissue separation of plant endophytes, but its potential impact on the composition and diversity of endophytes has rarely been investigated. In the present work, the influence of sodium hypochlorite (NaClO) on the diversity of endophytic bacteria and fungi in leaves and stems of tea plants was investigated. We found that the diversity of bacterial endophytes was significantly affected by the concentration of NaClO as well as the pretreatment time. Pretreatment with 0.5% NaClO for 8 min and 2.0% NaClO for 3 min were suitable for the tea plant leaves and stems, respectively, but the effects of NaClO on the diversity of fungal endophytes were limited according to the results from HTS. Regardless of NaClO sterilization, most of the endophytes in tissues, such as the dominant taxa, could not be Isolated by using the regular culture-dependent approaches. Collectively, our results demonstrated that the pretreatment with NaClO should be modified to precisely understand the diversity of endophytes from different tissues of tea plants and also indicate that more attention should be paid to establish specific culture-dependent protocols for the isolation of plant endophytes.
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