ArticleFrontiers in Marine Science2020
Atlantic Horseshoe Crabs and Endotoxin Testing: Perspectives on Alternatives, sustainable Methods, and the 3Rs (Replacement, Reduction, and Refinement).
Article in Frontiers in Marine Science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Comparison of PBS-Caffeine and Caffeine Buffers for Inhibiting Exocytosis During Horseshoe Crab Blood Collection and Improving the Yield of Limulus Amebocyte Lysate (LAL) for Endotoxin Detection.International journal of molecular sciences · 2026Article
- Systemic Evaluation of Administered FITC-Dextran or Evans Blue Dye to Determine Intestinal Barrier Permeability in Nonhuman Primates.Pathogens & immunity · 2026Article
- Chitosan in regenerative medicine: a translational perspective on biomaterial design.Frontiers in bioengineering and biotechnology · 2026Review
- Optimal Horseshoe Crab Blood Collection Solution That Inhibits Cellular Exocytosis and Improves Production Yield of Limulus Amoebocyte Lysate for Use in Endotoxin Tests.International journal of molecular sciences · 2025Article
- Isolator-based point-of-care manufacturing: a practical solution for GMP-compliant cell and extracellular vesicles therapy production.Frontiers in bioengineering and biotechnology · 2025Review
- The yield of Limulus amoebocyte lysate (LAL), which is used for endotoxin detection and adjunct diagnosis of invasive fungal infection, was optimized with caffeine collection buffer.Frontiers in microbiology · 2025Article
- Lessons for the Future of NAMs from History, Philosophy and Social Studies of Science.Alternatives to laboratory animals : ATLA · 2024Review
- Behavioural Indicators of Pain and Suffering in Arthropods and Might Pain Bite Back?Animals : an open access journal from MDPI · 2023Review
- Feral pharmaceuticalization-Biomedical uses of animal life in light of the global donkey hide trade.BioSocieties · 2023Article
- Wettability-based ultrasensitive detection of amphiphiles through directed concentration at disordered regions in self-assembled monolayers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation.Engineering in life sciences · 2022Article
- Edge cases in animal research law: Constituting the regulatory borderlands of the UK's Animals (Scientific Procedures) Act.Studies in history and philosophy of science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Endotoxin testing is a vital part of quality and safety control in pharmaceutical production. The primary method for this testing in North America and Europe is the limulus amebocyte lysate (LAL) test, a critical component of which is the blood of Atlantic horseshoe crabs (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.