Evidence map›Paper›PMID 35590240›Full record

ArticleBMC genomic data2022

Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing.

Xin Zhao, Wenpeng Liu, Baowang Liu, Qiang Zeng, Ziqiang Cui, Yang Wang, Jinglin Cao, Qingjun Gao, Caiyan Zhao, Jian Dou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomic data, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Xin ZhaoDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Wenpeng LiuDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Baowang LiuDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Qiang ZengDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Ziqiang CuiDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Yang WangDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Jinglin CaoDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Qingjun GaoDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China.
Caiyan ZhaoDepartment of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China.
Jian DouDepartment of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang City, 050051, Hebei Province, China. DouJian_doctor@163.com.
Hebei Medical University · CNThird Hospital of Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of our study was to use the differentially expressed mRNAs (DEmRNAs) and differentially expressed miRNAs (DEmiRNAs) to illustrate the underlying mechanism of hypoxia in liver cancer.

methodsIn this study, a cell model of hypoxia was established, and autophagy activity was measured with western blotting and transmission electron microscopy. The effect of hypoxia conditions on the invasion of liver cancer cell was evaluated. RNA sequencing was used to identify DEmRNAs and DEmiRNAs to explore the mechanism of hypoxia in liver cancer cells.

resultsWe found that autophagy activation was triggered by hypoxia stress and hypoxia might promote liver cancer cell invasion. In addition, a total of 407 shared DEmRNAs and 57 shared DEmiRNAs were identified in both HCCLM3 hypoxia group and SMMC-7721 hypoxia group compared with control group. Furthermore, 278 DEmRNAs and 24 DEmiRNAs were identified as cancer hypoxia-specific DEmRNAs and DEmiRNAs. Finally, we obtained 19 DEmiRNAs with high degree based on the DEmiRNA-DEmRNA interaction network. Among them, hsa-miR-483-5p, hsa-miR-4739, hsa-miR-214-3p and hsa-miR-296-5p may be potential gene signatures related to liver cancer hypoxia.

conclusionsOur study may help to understand the potential molecular mechanism of hypoxia in liver cancer.

Indexed as

Liver NeoplasmsMicroRNAsComputational BiologyHumansHypoxiaRNA, MessengerSequence Analysis, RNAMicroRNAsMIRN214 microRNA, humanMIRN296 microRNA, humanRNA, MessengerHypoxiaLiver cancerMicroRNAsRNA sequencing

Identifiers

PMID35590240
PMCPMC9121577
OpenAlexW4280547939

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.