ArticleBMC microbiology2022
Genomic analysis of qnr-harbouring IncX plasmids and their transferability within different hosts under induced stress.
Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Soil to host environmental determinants fueling horizontal gene transfer and global AMR dissemination.Folia microbiologica · 2026Review
- Dynamic changes in the plasmidome and resistome in the gastrointestinal tract of chickens.Microbiology spectrum · 2026Article
- Fluoroquinolone resistance in ESKAPE pathogens: evolutionary pathways, one health transmission, and clinical surveillance.Frontiers in microbiology · 2025Review
- Phenotypic and Genomic Characterization of ESBL- and AmpC-β-Lactamase-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-ResistantPathogens (Basel, Switzerland) · 2024Article
- First Data on WGS-Based Typing and Antimicrobial Resistance of HumanAntibiotics (Basel, Switzerland) · 2024Article
- Comparison of the antibiotic resistance mechanisms in a gram-positive and a gram-negative bacterium by gene networks analysis.PloS one · 2024Article
- Improvement of the conjugation transfer of N. gerenzanensis based on the synergistic effect of quorum sensing and antibiotic interference.AMB Express · 2023Article
- Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-ResistantMicroorganisms · 2023Article
- Quinolone Resistance Genes and Their Contribution to Resistance inAntibiotics (Basel, Switzerland) · 2023Article
- Klebsiella pneumoniae ST147: and then there were three carbapenemases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConjugative plasmids play a major role in the dissemination of antibiotic resistance genes. Knowledge of the plasmid characteristics and behaviour can allow development of control strategies. Here we focus on the IncX group of plasmids carrying genes conferring quinolone resistance (PMQR), reporting their transfer and persistence within host bacteria of various genotypes under distinct conditions and levels of induced stress in form of temperature change and various concentrations of ciprofloxacin supplementation.
methodsComplete nucleotide sequences were determined for eight qnr-carrying IncX-type plasmids, of IncX1 (3), IncX2 (3) and a hybrid IncX1-2 (2) types, recovered from Escherichia coli of various origins. This data was compared with further complete sequences of IncX1 and IncX2 plasmids carrying qnr genes (n = 41) retrieved from GenBank and phylogenetic tree was constructed. Representatives of IncX1 (pHP2) and IncX2 (p194) and their qnrS knockout mutants, were studied for influence of induced stress and genetic background on conjugative transfer and maintenance.
resultsA high level of IncX core-genome similarity was found in plasmids of animal, environmental and clinical origin. Significant differences were found between the individual IncX plasmids, with IncX1 subgroup plasmids showing higher conjugative transfer rates than IncX2 plasmids. Knockout of qnr modified transfer frequency of both plasmids. Two stresses applied simultaneously were needed to affect transfer rate of wildtype plasmids, whereas a single stress was sufficient to affect the IncX ΔqnrS plasmids. The conjugative transfer was shown to be biased towards the host phylogenetic proximity. A long-term cultivation experiment pointed out the persistence of IncX plasmids in the antibiotic-free environment.
conclusionsThe study indicated the stimulating effect of ciprofloxacin supplementation on the plasmid transfer that can be nullified by the carriage of a single PMQR gene. The findings present the significant properties and behaviour of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination and stability in bacterial populations.
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