Evidence map›Paper›PMID 35589966›Full record

ArticleThe ISME journal2022

Heme cross-feeding can augment Staphylococcus aureus and Enterococcus faecalis dual species biofilms.

Jun-Hong Ch'ng, Mugil Muthu, Kelvin K L Chong, Jun Jie Wong, Casandra A Z Tan, Zachary J S Koh, Daniel Lopez, Artur Matysik, Zeus J Nair, Timothy Barkham and 2 more

Open access · hybridAbstract read
In one paragraph

Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 69 citations in OpenAlex.

  1. InJournal of bacteriology · 2026
    Article
  2. Article
  3. Journal of bacteriology · 2026
    Article
  4. InbioRxiv : the preprint server for biology · 2026
    Article
  5. Review
  6. Article
  7. Article
  8. Infection and immunity · 2026
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. In vivo virulence ofJTCVS open · 2025
    Article
  16. Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025
    Review
  17. Genome-wide analysis ofJournal of bacteriology · 2025
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Jun-Hong Ch'ngDepartment of Microbiology and Immunology, National University of Singapore, Singapore, Singapore. micchn@nus.edu.sg.ORCID http://orcid.org/0000-0002-1143-5611
Mugil MuthuDepartment of Microbiology and Immunology, National University of Singapore, Singapore, Singapore.
Kelvin K L ChongSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.
Jun Jie WongSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.ORCID http://orcid.org/0000-0002-9455-1051
Casandra A Z TanSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.ORCID http://orcid.org/0000-0002-4637-488X
Zachary J S KohDepartment of Microbiology and Immunology, National University of Singapore, Singapore, Singapore.
Daniel LopezSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.
Artur MatysikSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.ORCID http://orcid.org/0000-0003-3702-9491
Zeus J NairSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.ORCID http://orcid.org/0000-0001-9662-4958
Timothy BarkhamDepartment of Microbiology and Immunology, National University of Singapore, Singapore, Singapore.ORCID http://orcid.org/0000-0003-0975-2244
Yulan WangSingapore Phenome Center, Lee Kong Chian School of Medicine, Nanyang Technological University, Nanyang, Singapore.ORCID http://orcid.org/0000-0002-2831-8737
Kimberly A KlineSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore. kkline@ntu.edu.sg.ORCID http://orcid.org/0000-0002-5472-3074
Nanyang Technological University · SGNational University of Singapore · SG

Funding

Ministry of Education - Singapore (MOE) SCELSENational Research Foundation Singapore (National Research Foundation-Prime Minister's office, Republic of Singapore) SCELSE
6 · The paper itself

Abstract

The contribution of biofilms to virulence and as a barrier to treatment is well-established for Staphylococcus aureus and Enterococcus faecalis, both nosocomial pathogens frequently isolated from biofilm-associated infections. Despite frequent co-isolation, their interactions in biofilms have not been well-characterized. We report that in combination, these two species can give rise to augmented biofilms biomass that is dependent on the activation of E. faecalis aerobic respiration. In E. faecalis, respiration requires both exogenous heme to activate the cydAB-encoded heme-dependent cytochrome bd, and the availability of O

Indexed as

Enterococcus faecalisStaphylococcus aureusBiofilmsHemeVirulenceHeme

Identifiers

PMID35589966
PMCPMC9296619
OpenAlexW4280635279

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.