Evidence map›Paper›PMID 35588643›Full record

ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2022

Suspect screening of exogenous compounds using multiple reaction screening (MRM) profiling in human urine samples.

César A Marasco Júnior, Madison E Edwards, Rafaela S Lamarca, Tiago J P Sobreira, Jeffrey M Caterino, David S Hains, Andrew L Schwaderer, Paulo Clairmont F de Lima Gomes, Christina R Ferreira

Abstract read
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In one paragraph

Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

César A Marasco JúniorNational Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Institute of Chemistry, São Paulo State University (UNESP), P.O. Box 355, 14800-060 Araraquara, São Paulo, Brazil; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Madison E EdwardsDepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Rafaela S LamarcaNational Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Institute of Chemistry, São Paulo State University (UNESP), P.O. Box 355, 14800-060 Araraquara, São Paulo, Brazil.
Tiago J P SobreiraBindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA.
Jeffrey M CaterinoDepartment of Emergency Medicine, The Ohio State University, Columbus, OH 43210, USA.
David S HainsDepartment of Pediatrics, Indiana University, Indianapolis, IN 43202, USA.
Andrew L SchwadererDepartment of Pediatrics, Indiana University, Indianapolis, IN 43202, USA.
Paulo Clairmont F de Lima GomesNational Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Institute of Chemistry, São Paulo State University (UNESP), P.O. Box 355, 14800-060 Araraquara, São Paulo, Brazil. Electronic address: paulo.clairmont@unesp.br.
Christina R FerreiraDepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USA; Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA. Electronic address: cferrei@purdue.edu.

Funding

Urine antimicrobial proteins in older adults: aging, infection, & innate immunityR01AG050801 · NIA · OHIO STATE UNIVERSITY · PI CATERINO, JEFFREY M · 2016 to 2020
$2.8M
Genetic Regulation, Tubular Processing and Clinical Relevance of Collecting Duct alpha-Defensins 1-3R01DK117934 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI CINES, DOUGLAS BROCK, HAINS, DAVID SULLIVAN · 2018 to 2021
$2.3M
The interface between critical acid-base mediators and the renal bacterial defenseR01DK106286 · NIDDK · RESEARCH INST NATIONWIDE CHILDREN'S HOSP · PI HAINS, DAVID SULLIVAN, SCHWADERER, ANDREW LAWRENCE · 2015 to 2018
$1.6M
NIA NIH HHS R01 AG050801NIDDK NIH HHS R01 DK106286
6 · The paper itself

Abstract

Thousands of chemical compounds produced by industry are dispersed in the human environment widely enough to reach the world population, and the introduction of new chemicals constantly occurs. As new synthetic molecules emerge, rapid analytical workflows for screening possible presence of exogenous compounds in biofluids can be useful as a first pass analysis to detect chemical exposure and guide the development and application of more elaborate LC-MS/MS methods for quantification. In this study, a suspect screening workflow using the multiple reaction monitoring (MRM) profiling method is proposed as a first pass exploratory technique to survey selected exogenous molecules in human urine samples. The workflow was applied to investigate 12 human urine samples using 310 MRMs related to the chemical functionalities of 87 exogenous compounds present in the METLIN database and reported in the literature. A total of 11 MRMs associated with five different compounds were detected in the samples. Product ion scans for the precursor ions of the selected MRMs were acquired as a further identification step for these chemicals. The suspect screening results suggested the presence of five exogenous compounds in the human urine samples analyzed, namely metformin, metoprolol, acetaminophen, paraxanthine and acrylamide. LC-MS/MS was applied as a last step to confirm these results, and the presence of four out of the five targets selected by MRM profiling were corroborated, indicating that this workflow can support the selection of suspect compounds to screen complex samples and guide more time-consuming and specific quantification analyses.

Indexed as

Tandem Mass SpectrometryChromatography, LiquidDatabases, FactualHumansWorkflowExposomeMRM profilingSuspect screening method

Identifiers

PMID35588643
PMCPMC10566365

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.