ArticleeLife2022
Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.
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Who cites it
85 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Meta-analysis of gonadal transcriptome provides novel insights into sex change mechanism across protogynous fishes.Genes to cells : devoted to molecular & cellular mechanisms · 2024Pooled it
- MEIOSIN retains a STRA8-independent activity that contributes to meiotic gene activation across vertebrates.Genes & development · 2026Article
- Short-term gonadal cultures are sufficient for germline transmission in a songbird.Stem cell reports · 2026Article
- Long-term rock dove (Columba livia) primordial germ cell culture: A tool towards avian conservation.The Journal of heredity · 2026Article
- Ultra-fast generation of all-female grass carp via transplantation of female germline stem cells into zebrafish.Science China. Life sciences · 2026Article
- Functional Sex Reversal in Adult Gonochoristic Teleosts is Mediated by Ovarian Germline Stem Cell Plasticity and Somatic Cell Transdifferentiation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-Cell RNA-Seq of Pituitary and Ovary Identifies Regulators of Reproduction in Yellow Catfish (Animals : an open access journal from MDPI · 2026Article
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Molecular evidence for early deuterostome origins of ovarian cell types and neuroendocrine control of reproduction.Nature communications · 2026Article
- Stn1 promotes zebrafish oocyte development via amplifying Wnt/β-catenin signaling.EMBO reports · 2026Article
- dUTPase is essential in zebrafish development and possesses several single-nucleotide variants with pronounced structural and functional consequences.FEBS open bio · 2026Article
- Single-Nucleus RNA-Seq Reveals Neuroprotective Effects of Acupuncture in Chronic Migraine Through Modulation of Glial Subtypes.CNS neuroscience & therapeutics · 2026Article
- Evaluating the Effects of Neburon Exposure on Ovarian Folliculogenesis Using Zebrafish and Mouse Granulosa Cell Line.Environmental health perspectives · 2026Article
- Comparative single-cell analysis of black rockfish (Sebastes schlegelii) ovarian stroma reveals placenta-like adaptations toward viviparity.Communications biology · 2026Article
- Cross-species single-cell transcriptomic analyses reveal evolutionary conservation and diversification of ovarian tissues.Journal of animal science and biotechnology · 2026Article
- Evaluation of season and sex specific differential gonadal transcriptomics in Himalayan snow trout Schizothorax plagiostomus, Heckel, 1838.Scientific reports · 2026Article
- Sex-Specific Gene Expression Ontogeny During Gonadal Development in Post-Metamorphic Xenopus tropicalis.Molecular reproduction and development · 2026Article
- Tdrd6a and Tdrd6b are required together for germ plasm formation.Development (Cambridge, England) · 2026Article
- Single-nucleus RNA sequencing reveals the underlying roadmap of early gonadal differentiation in teleost.Science China. Life sciences · 2026Article
- Genetic and functional characterization of AMH Signaling in Zebrafish - Evidence for Roles of Amh-Bmpr2a-Bmpr1bb Pathway in Controlling Gonadal Homeostasis.PLoS genetics · 2026Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Zebrafish are an established research organism that has made many contributions to our understanding of vertebrate tissue and organ development, yet there are still significant gaps in our understanding of the genes that regulate gonad development, sex, and reproduction. Unlike the development of many organs, such as the brain and heart that form during the first few days of development, zebrafish gonads do not begin to form until the larval stage (≥5 days post-fertilization). Thus, forward genetic screens have identified very few genes required for gonad development. In addition, bulk RNA-sequencing studies that identify genes expressed in the gonads do not have the resolution necessary to define minor cell populations that may play significant roles in the development and function of these organs. To overcome these limitations, we have used single-cell RNA sequencing to determine the transcriptomes of cells isolated from juvenile zebrafish ovaries. This resulted in the profiles of 10,658 germ cells and 14,431 somatic cells. Our germ cell data represents all developmental stages from germline stem cells to early meiotic oocytes. Our somatic cell data represents all known somatic cell types, including follicle cells, theca cells, and ovarian stromal cells. Further analysis revealed an unexpected number of cell subpopulations within these broadly defined cell types. To further define their functional significance, we determined the location of these cell subpopulations within the ovary. Finally, we used gene knockout experiments to determine the roles of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.