ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2022
Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Recombinant ubbR promoter enables highly efficient tamoxifen-inducible Cre recombination in embryonic and adult zebrafish.Genetics · 2026Article
- Zebrafish Ace2N-mNeon expression toolkit forNeurophotonics · 2026Article
- Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Long-term labelling and tracing of endodermal cells using a perpetual cycling Gal4-UAS system.Development (Cambridge, England) · 2025Article
- Lineage labeling with zebrafishbioRxiv : the preprint server for biology · 2024Article
- Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase.Biology open · 2024Article
- Multiple embryonic sources converge to form the pectoral girdle skeleton in zebrafish.Nature communications · 2024Article
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- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
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10 authors.
Funding
Abstract
backgroundThe most-common strategy for zebrafish Cre/lox-mediated lineage labeling experiments combines ubiquitously expressed, lox-based Switch reporter transgenes with tissue-specific Cre or 4-OH-Tamoxifen-inducible CreERT2 driver lines. Although numerous Cre driver lines have been produced, only a few broadly expressed Switch reporters exist in zebrafish and their generation by random transgene integration has been challenging due to position-effect sensitivity of the lox-flanked recombination cassettes. Here, we compare commonly used Switch reporter lines for their recombination efficiency and reporter expression pattern during zebrafish development.
resultsUsing different experimental setups, we show that ubi:Switch and hsp70l:Switch outperform current generations of the two additional Switch reporters actb2:BFP-DsRed and actb2:Stop-DsRed. Our comparisons also document preferential Cre-dependent recombination of ubi:Switch and hsp70l:Switch in distinct zebrafish tissues at early developmental stages. To investigate what genomic features may influence Cre accessibility and lox recombination efficiency in highly functional Switch lines, we mapped these transgenes and charted chromatin dynamics at their integration sites.
conclusionsOur data documents the heterogeneity among lox-based Switch transgenes toward informing suitable transgene selection for lineage labeling experiments. Our work further proposes that ubi:Switch and hsp70l:Switch define genomic integration sites suitable for universal transgene or switch reporter knock-in in zebrafish.
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