Evidence map›Paper›PMID 35581482›Full record

ArticleMedical oncology (Northwood, London, England)2022

Expression of proliferation-related genes in BM-MSC-treated ALL cells in hypoxia condition is regulated under the influence of epigenetic factors in-vitro.

Xiaoyu Yang, Yan Wang, Heshu Sulaiman Rahman, Talar Ahmad Merza Mohammad, Amin Daei Sorkhabi, Stanislav Evgenievich Korsakov, Lakshmi Thangavelu, Ali Adili, Aila Sarkesh, Rozita Tamjidifar and 4 more

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In one paragraph

Article in Medical oncology (Northwood, London, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 5 countries.

Xiaoyu YangDepartment of Hematology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, 450052, Henan Province, China.
Yan WangDepartment of Hematology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, 450052, Henan Province, China.
Heshu Sulaiman RahmanDepartment of Physiology, College of Medicine, University of Sulaimani, Sulaimaniyah, Iraq.
Talar Ahmad Merza MohammadDepartment of Pharmacology and Toxicology, College of Pharmacy, HMU (Hawler Medical University), Erbil, Iraq.
Amin Daei SorkhabiMedical Laboratory Analysis Department, College of Health Sciences, Cihlan University of Sulaimaniya, Kurdistan Region, Iraq.
Stanislav Evgenievich KorsakovDepartment of maxillofacial surgery, I. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Lakshmi ThangaveluDepartment of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, India.
Ali AdiliDepartment of Oncology, Tabriz University of Medical Sciences, Tabriz, Iran.
Aila SarkeshMedical Laboratory Analysis Department, College of Health Sciences, Cihlan University of Sulaimaniya, Kurdistan Region, Iraq.
Rozita TamjidifarImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Hossein SaeediImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Ramin AslaminabadImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Saeed TarziImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Morteza AkbariImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. akbarimo@tbzmed.ac.ir.ORCID http://orcid.org/0000-0001-7333-1300
Tabriz University of Medical Sciences · IRFifth Affiliated Hospital of Zhengzhou University · CNHawler Medical University · IQSaveetha University · INSechenov University · RUUniversity of Sulaimani · IQ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells affect ALL cell biology under hypoxic conditions. We studied survival, proliferation, expression, and promoter methylation levels of essential genes involved in expanding MOLT-4 cells co-cultured with BM-MSC under the hypoxic condition. Here, MOLT-4 cells were co-cultured with BMMSCs under hypoxic conditions. First, the apoptosis rate was evaluated by Flow cytometry. Then, MOLT-4 cells' proliferation rate was assessed using MTT assay, and the expressions and methylation rates of genes were determined by qRT-PCR and MS-qPCR, respectively. The results showed that although MOLT-4 cells proliferation and survival rates were reduced under hypoxic conditions, this reduction was not statistically significant. Also, we showed that hypoxic conditions caused upregulation of candidate genes and affected their methylation status. Besides, it was revealed that Pontin was downregulated, while KDM3A, SKP2, and AURKA had an upward trend in the presence of MOLT-4 cells plus BM-MSC. The co-culture of leukemia cells with BMMSCs under hypoxic conditions may be a potential therapeutic approach for ALL.

Indexed as

Mesenchymal Stem CellsApoptosisCell HypoxiaCell ProliferationCells, CulturedEpigenesis, GeneticHumansHypoxiaJumonji Domain-Containing Histone DemethylasesJumonji Domain-Containing Histone DemethylasesKDM3A protein, humanALLBMMSCsHypoxiaMethylationPontin

Identifiers

PMID35581482
OpenAlexW4280528957

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.