Evidence map›Paper›PMID 35581427›Full record

ArticleAnalytical and bioanalytical chemistry2022

Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence.

Kari Kopra, Nazia Hassan, Emmiliisa Vuorinen, Salla Valtonen, Randa Mahran, Huda Habib, Pinja Jalkanen, Petri Susi, Vesa Hytönen, Minna Hankaniemi and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Fluorescence-Based Protein Stability Monitoring-A Review.International journal of molecular sciences · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Kari Kopra *Department of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Nazia Hassan *Department of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Emmiliisa VuorinenDepartment of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Salla ValtonenDepartment of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Randa MahranDepartment of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Huda HabibDepartment of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland.
Pinja JalkanenInstitute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland.
Petri SusiInstitute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland.
Vesa HytönenFaculty of Medicine and Health Technology, Tampere University, 33014, Tampere, Finland.
Minna HankaniemiFaculty of Medicine and Health Technology, Tampere University, 33014, Tampere, Finland.
Seppo Ylä-HerttualaA.I.Virtanen Institute, University of Eastern Finland and Gene Therapy Unit, Kuopio University Hospital, 70210, Kuopio, Finland.
Laura KakkolaInstitute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland.
Markus PeurlaInstitute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland.
Harri HärmäDepartment of Chemistry, University of Turku, Henrikinkatu 2, 20500, Turku, Finland. harri.harma@utu.fi.
University of Turku · FITampere University · FIKuopio University Hospital · FI

Funding

Academy of Finland 296093Academy of Finland 309455Academy of Finland 323433Academy of Finland 329012
6 · The paper itself

Abstract

Viruses play a major role in modern society and create risks from global pandemics and bioterrorism to challenges in agriculture. Virus infectivity assays and genome copy number determination methods are often used to obtain information on virus preparations used in diagnostics and vaccine development. However, these methods do not provide information on virus particle count. Current methods to measure the number of viral particles are often cumbersome and require highly purified virus preparations and expensive instrumentation. To tackle these problems, we developed a simple and cost-effective time-resolved luminescence-based method for virus particle quantification. This mix-and-measure technique is based on the recognition of the virus particles by an external Eu

Indexed as

Virus DiseasesVirusesHumansLuminescenceVirionLabel-freeProtein-ProbeTime-resolved luminescenceVirus particle quantification

Identifiers

PMID35581427
PMCPMC9113738
OpenAlexW4280579741

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.