ArticleProceedings of the National Academy of Sciences of the United States of America2022
Focused ultrasound excites action potentials in mammalian peripheral neurons in part through the mechanically gated ion channel PIEZO2.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 91 citations in OpenAlex.
- Piezo2FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Focused ultrasound for cellular mechanoactivation and nanomedicine delivery in cancer.Biomedical microdevices · 2026Review
- Displacement-Guided Focused Ultrasound of the Median Nerve Modulates Somatosensory Evoked Potentials in Humans.IEEE transactions on bio-medical engineering · 2026Article
- Molecular and biophysical characterization of cultured DRG neurons in response to focused ultrasound.Scientific reports · 2026Article
- Channel-specific differential effects of bacterial mechanosensitive channels for ultrasound neuromodulation in precision sonogenetics.Theranostics · 2026Article
- Targeting Piezo1/2 ion channels for modulation of the lymphatic system: mechanisms, applications, and therapeutic prospects.Burns & trauma · 2026Review
- Discovery Pipeline for Acute Kidney Injury: Molecules, Mechanisms, Models, and Targets.Nephron · 2026Review
- Mechanically gated currents in mouse sensory neurons lacking PIEZO2.Biophysical journal · 2025Article
- Challenges and lessons learned in attempts to develop in vitro models for ultrasound neuromodulation using local field potential measurements in hippocampal tissue.Biophysical journal · 2025Article
- Spinal cord neuromodulation for blood pressure control using low-intensity focused ultrasound.Scientific reports · 2025Article
- MDFIC2 is a PIEZO channel modulator that can alleviate mechanical allodynia associated with neuropathic pain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Low-Intensity ultrasound for controlled excitation and suppression in rat sciatic nerve.Scientific reports · 2025Article
- Merkel cell stimulation in fear and sensory signaling.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2025Article
- Trauma and Sensory Systems: Biological Mechanisms Involving the Skin and the 17q21 Gene Cluster.Biological psychiatry · 2025Review
- Individualized non-invasive deep brain stimulation of the basal ganglia using transcranial ultrasound stimulation.Nature communications · 2025Article
- Focused Ultrasound Modulates Dopamine in a Mesolimbic Reward Circuit.Journal of neurochemistry · 2025Article
- Parallelized Mechanical Stimulation of Neuronal Calcium Through Cell-Internal Nanomagnetic Forces Provokes Lasting Shifts in the Network Activity State.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Optimized ultrasound neuromodulation for non-invasive control of behavior and physiology.Neuron · 2024Article
- Piezo2 voltage-block regulates mechanical pain sensitivity.Brain : a journal of neurology · 2024Article
- Mechanosensitive ion channels in glaucoma pathophysiology.Vision research · 2024Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Neurons of the peripheral nervous system (PNS) are tasked with diverse roles, from encoding touch, pain, and itch to interoceptive control of inflammation and organ physiology. Thus, technologies that allow precise control of peripheral nerve activity have the potential to regulate a wide range of biological processes. Noninvasive modulation of neuronal activity is an important translational application of focused ultrasound (FUS). Recent studies have identified effective strategies to modulate brain circuits; however, reliable parameters to control the activity of the PNS are lacking. To develop robust noninvasive technologies for peripheral nerve modulation, we employed targeted FUS stimulation and electrophysiology in mouse ex vivo skin-saphenous nerve preparations to record the activity of individual mechanosensory neurons. Parameter space exploration showed that stimulating neuronal receptive fields with high-intensity, millisecond FUS pulses reliably and repeatedly evoked one-to-one action potentials in all peripheral neurons recorded. Interestingly, when neurons were classified based on neurophysiological properties, we identified a discrete range of FUS parameters capable of exciting all neuronal classes, including myelinated A fibers and unmyelinated C fibers. Peripheral neurons were excited by FUS stimulation targeted to either cutaneous receptive fields or peripheral nerves, a key finding that increases the therapeutic range of FUS-based peripheral neuromodulation. FUS elicited action potentials with millisecond latencies compared with electrical stimulation, suggesting ion channel–mediated mechanisms. Indeed, FUS thresholds were elevated in neurons lacking the mechanically gated channel PIEZO2. Together, these results demonstrate that transcutaneous FUS drives peripheral nerve activity by engaging intrinsic mechanotransduction mechanisms in neurons [B. U. Hoffman, PhD thesis, (2019)].
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