Evidence map›Paper›PMID 35579756›Full record

ArticleParasitology research2022

Prevalence and genetic characterization of Enterocytozoon bieneusi in children in Northeast Egypt.

Doaa Naguib, Dawn M Roellig, Nagah Arafat, Lihua Xiao

Open access · greenAbstract read
In one paragraph

Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Molecular epidemiology ofFrontiers in cellular and infection microbiology · 2026
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  5. Article
  6. Prevalence and molecular characterization ofFrontiers in veterinary science · 2025
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  7. Article
  8. Molecular Detection ofMicroorganisms · 2024
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  9. Molecular detection and characterization ofFrontiers in veterinary science · 2023
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Doaa NaguibDepartment of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Dawn M RoelligDivision of Foodborne, Centers for Disease Control and Prevention, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Atlanta, GA, 30329, USA.
Nagah ArafatDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Lihua XiaoCenter for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. lxiao1961@gmail.com.ORCID http://orcid.org/0000-0001-8532-2727
Mansoura University · EGCenters for Disease Control and Prevention · USSouth China Agricultural University · CN

Funding

Higher Education Discipline Innovation Project D20008Intramural CDC HHS CC999999National Natural Science Foundation of China 31820103014
6 · The paper itself

Abstract

Enterocytozoon bieneusi is the most common microsporidia in humans worldwide, in addition to infecting a wide range of animals. However, there is limited information about this pathogen in children in Egypt. Here, we carried out a molecular epidemiological study of E. bieneusi in child care centers in three provinces in Egypt. Altogether, 585 fresh fecal samples were collected from children attending 18 child care centers in El-Dakahlia, El-Gharbia, and Damietta provinces in Northeast Egypt during March 2015 to April 2016. PCR and sequence analyses of the ribosomal internal transcribed spacer (ITS) were used to detect and genotype E. bieneusi. Twenty-seven fecal samples (4.6%, 27/585) were positive for E. bieneusi. Five genotypes were identified, including type IV (n = 13), Peru8 (n = 9), Peru6 (n = 2), Peru11 (n = 2), and D (n = 1). Phylogenetic analysis indicated that the five genotypes of E. bieneusi detected in this study were clustered into zoonotic group 1. These data provide important information on the prevalence and genetic diversity of E. bieneusi in children in this country. Further epidemiological studies should be conducted to elucidate the role of zoonotic transmission in human E. bieneusi infections.

Indexed as

EnterocytozoonMicrosporidiosisAnimalsChinaEgyptFecesGenetic VariationGenotypeHumansPhylogenyPrevalenceSequence Analysis, DNAZoonosesChildrenEgyptEnterocytozoon bieneusiGenotypesInternal transcribed spacerZoonoses

Identifiers

PMID35579756
PMCPMC9218962
OpenAlexW4280573299

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.