Evidence map›Paper›PMID 35578733›Full record

ArticleSTAR protocols2022

Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape.

Naveenchandra Suryadevara, Pavlo Gilchuk, Seth J Zost, Nikhil Mittal, Li Leyna Zhao, James E Crowe, Robert H Carnahan

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Naveenchandra SuryadevaraVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Pavlo GilchukVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Seth J ZostVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Nikhil MittalAgilent Technology, Inc., 6779 Mesa Ridge Road, Suite 100, San Diego, CA 92121, USA.
Li Leyna ZhaoAgilent Technology, Inc., 6779 Mesa Ridge Road, Suite 100, San Diego, CA 92121, USA.
James E CroweVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Robert H CarnahanVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Funding

B-CELL EPITOPE DISCOVERY AND MECHANISMS OF ANTIBODY PROTECTION: Modification for SARS-CoV2 Research75N93019C00074 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI CROWE, JAMES E · 2019 to 2024
$10.2M
Human antibody-based countermeasures against the Wuhan Coronavirus SARS-CoV-2R01AI157155 · NIAID · WASHINGTON UNIVERSITY · PI BARIC, RALPH S, CROWE, JAMES E · 2020 to 2024
$6.0M
NIAID NIH HHS 75N93019C00074NIAID NIH HHS R01 AI157155
6 · The paper itself

Abstract

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021).

Indexed as

COVID-19SARS-CoV-2Antibodies, MonoclonalAntibodies, ViralHumansSpike Glycoprotein, CoronavirusAntibodies, MonoclonalAntibodies, ViralSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2AntibodyBiotechnology and bioengineeringCell-based AssaysHealth SciencesHigh Throughput ScreeningImmunologyMicrobiology

Identifiers

PMID35578733
PMCPMC9023333

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.