ArticlePest management science2022
Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees.
Article in Pest management science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- RNAi-Based Pesticides: Genetic Innovations for Sustainable Crop Protection and One Health.Advanced genetics (Hoboken, N.J.) · 2026Article
- Variability in mountain pine beetle (Coleoptera: Curculionidae) gene expression in response to increasing concentrations of species-specific double-stranded RNA.Journal of insect science (Online) · 2026Article
- Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.The plant pathology journal · 2025Review
- Inhibition of Hop Stunt Viroid by Exogenous Double-Stranded RNA in Micropropagated Grapevine Plantlets.The plant pathology journal · 2025Article
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Review
- Review
- Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants.Journal of nanobiotechnology · 2024Article
- RNAi-based pest control: Production, application and the fate of dsRNA.Frontiers in bioengineering and biotechnology · 2022Review
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundRNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double-stranded RNA (dsRNA), and season-long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).
resultsThe qRT-PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 pg/30 mg of leaf tissue, and in 2020 was 16.4 pg/30 mg. The persistence of dsRNA in the apple tree canopy in 2019 was at least 84 days, and in 2020 was at least 141 days.
conclusionsThe highest mean measurement of dsRNA on a single date in 2019 was 242 pg, which is approximately equivalent to 8 ng/1 g leaf tissue. The projection using the highest replicate concentration from the same date is approximately equivalent to 27 ng/1 g leaf tissue, which may be sufficient to be considered biologically active. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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Registered trials
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