Evidence map›Paper›PMID 35578348›Full record

ArticleStem cell research & therapy2022

Intramuscular delivery of neural crest stem cell spheroids enhances neuromuscular regeneration after denervation injury.

LeeAnn K Li, Wen-Chin Huang, Yuan-Yu Hsueh, Ken Yamauchi, Natalie Olivares, Raul Davila, Jun Fang, Xili Ding, Weikang Zhao, Jennifer Soto and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Therapeutic role of neural stem cells in neurological diseases.Frontiers in bioengineering and biotechnology · 2024
    Review
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 2 countries.

LeeAnn K LiDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Wen-Chin HuangDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Yuan-Yu HsuehDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Ken YamauchiDepartment of Neurobiology, University of California, Los Angeles, USA.
Natalie OlivaresDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Raul DavilaDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Jun FangDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Xili DingDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Weikang ZhaoDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Jennifer SotoDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Mahdi HasaniDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA.
Bennett NovitchDepartment of Neurobiology, University of California, Los Angeles, USA.
Song LiDepartments of Bioengineering and Department of Medicine, University of California, Los Angeles, USA. songli@ucla.edu.ORCID 0000-0002-4760-8828
University of California, Los Angeles · US

Funding

UCLA-Caltech Medical Scientist Training ProgramT32GM008042 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AJIJOLA, OLUJIMI A, DAWSON, DAVID WAYNE · 1985 to 2023
$29.9M
Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
PREPARATION AND DISTRUBUTION OF ADULT STEM CELLS: HUMAN &RODENTP40RR017447 · NCRR · TULANE UNIVERSITY OF LOUISIANA · PI PROCKOP, DARWIN JOHNSON · 2003 to 2011
$8.9M
Multimodal wireless electrical stimulation for tissue regenerationR01NS126918 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Song Li, John Rogers · 2022 to 2026
$2.4M
In Situ Regeneration of Blood VesselR01HL121450 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LI, SONG · 2015 to 2018
$1.8M
NCRR NIH HHS P40 RR017447NHLBI NIH HHS R01 HL121450NIDDK NIH HHS P30 DK041301NIGMS NIH HHS T32 GM008042NIH HHS GM008042NIH HHS HL121450NIH HHS NS126918NINDS NIH HHS R01 NS126918
6 · The paper itself

Abstract

backgroundMuscle denervation from trauma and motor neuron disease causes disabling morbidities. A limiting step in functional recovery is the regeneration of neuromuscular junctions (NMJs) for reinnervation. Stem cells have the potential to promote these regenerative processes, but current approaches have limited success, and the optimal types of stem cells remain to be determined. Neural crest stem cells (NCSCs), as the developmental precursors of the peripheral nervous system, are uniquely advantageous, but the role of NCSCs in neuromuscular regeneration is not clear. Furthermore, a cell delivery approach that can maintain NCSC survival upon transplantation is critical.

methodsWe established a streamlined protocol to derive, isolate, and characterize functional p75

resultsNCSCs cultured in spheroids displayed enhanced secretion of soluble factors involved in neuromuscular regeneration. Intramuscular transplantation of spheroids enabled long-term survival and retention of NCSCs, in contrast to the transplantation of single-cell suspensions. Furthermore, NCSC spheroids significantly improved functional recovery after four weeks as shown by gait analysis, electrophysiology, and the rate of NMJ innervation. MSC spheroids, on the other hand, had insignificant effect. In vitro co-culture of NCSC or MSC spheroids with engineered myotubes and motor neurons further evidenced improved innervated NMJ formation with NCSC spheroids.

conclusionsWe demonstrate that stem cell type is critical for neuromuscular regeneration and that NCSCs have a distinct advantage and therapeutic potential to promote reinnervation following peripheral nerve injury. Biophysical effects of spheroidal culture, in particular, enable long-term NCSC survival following in vivo delivery. Furthermore, synthetic neuromuscular tissue, or "tissues-on-a-chip," may offer a platform to evaluate stem cells for neuromuscular regeneration.

Indexed as

Induced Pluripotent Stem CellsNeural Stem CellsDenervationHumansNeural CrestNeurogenesisNeural crest stem cellNeuromuscular junctionOrgan-on-a-chipPeripheral nerve injuryRegenerative medicineSpheroidTissue engineering

Identifiers

PMID35578348
PMCPMC9109326
OpenAlexW4280509877

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.