ArticleNature methods2022
Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Review
- Aberration-aware 3D localization microscopy via self-supervised neural-physics learning.Nature communications · 2026Article
- Interferometric ultra-high resolution 3D imaging through brain sections.Nature communications · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Molecular-scale isotropic 3D super-resolution microscopy via interference localization.Nature methods · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Paralemmin-1 controls the nanoarchitecture of the neuronal submembrane cytoskeleton.Science advances · 2025Article
- Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.Nature methods · 2025Article
- Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view.bioRxiv : the preprint server for biology · 2024Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
- Article
- Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria.Cell death and differentiation · 2024Article
- 4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rhodamine-based fluorescent probe for dynamic STED imaging of mitochondria.Biomedical optics express · 2024Article
- Deep learning-driven adaptive optics for single-molecule localization microscopy.Nature methods · 2023Article
- Universal inverse modelling of point spread functions for SMLM localization and microscope characterization.bioRxiv : the preprint server for biology · 2023Article
- Enhancing obSTORM imaging performance with cubic spline PSF modeling.Biomedical optics express · 2023Article
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Authors and funding
12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10 nm spatial resolution in three dimensions. Despite its outstanding sensitivity, wider application of this technique has been hindered by complex instrumentation and the challenging nature of the data analysis. Here we report the development of a 4Pi-STORM microscope, which obtains optimal resolution and accuracy by modeling the 4Pi point spread function (PSF) dynamically while also using a simpler optical design. Dynamic spline PSF models incorporate fluctuations in the modulation phase of the experimentally determined PSF, capturing the temporal evolution of the optical system. Our method reaches the theoretical limits for precision and minimizes phase-wrapping artifacts by making full use of the information content of the data. 4Pi-STORM achieves a near-isotropic three-dimensional localization precision of 2-3 nm, and we demonstrate its capabilities by investigating protein and nucleic acid organization in primary neurons and mammalian mitochondria.
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