ArticleNature chemistry2022
Ultrafast proton-coupled isomerization in the phototransformation of phytochrome.
Article in Nature chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Polarization-resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume.FEBS open bio · 2026Article
- Deciphering the infrared spectrum of the hydrated proton using full-dimensional quantum dynamics.Nature chemistry · 2026Article
- Proton-Coupled Chromophore and Protein Structural Changes Control Phytochrome Activation.Biochemistry · 2026Article
- PHY domain governs structural and photochemical fidelity in the far-red-absorbing state of phytochromes.Frontiers in molecular biosciences · 2026Article
- The first microseconds of the life of excited heptamethine cyanine revealed by femtosecond stimulated Raman spectroscopy.Communications chemistry · 2025Article
- Photoisomerization of phytochrome's chromophore: a vibrational spectroscopic view on the primary ground state processes.RSC advances · 2025Article
- Chemical Mechanism of Allosteric and Asymmetric Dark Reversion in a Bacterial Phytochrome Uncovered by Cryo-EM.Journal of the American Chemical Society · 2025Article
- Ultrafast, remote-controlled protonation reaction enables structural changes in a phytochrome.Science advances · 2025Article
- A comparative study of femtosecond pulsed and continuous wave lasers on physiological responses through activation of phytochromes in seeds.Scientific reports · 2025Article
- Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes.Science advances · 2025Article
- Integrated Study of Fluorescence Enhancement in the Y176H Variant of Cyanobacterial Phytochrome Cph1.Biochemistry · 2025Article
- Hydrogen Bonding and Noncovalent Electric Field Effects in the Photoconversion of a Phytochrome.The journal of physical chemistry. B · 2024Article
- Analogies and Differences in the Photoactivation Mechanism of Bathy and Canonical Bacteriophytochromes Revealed by Multiscale Modeling.The journal of physical chemistry letters · 2024Article
- On the feasibility of time-resolved X-ray powder diffraction of macromolecules using laser-driven ultrafast X-ray sources.Journal of applied crystallography · 2024Article
- Photocobilins integrate BNature communications · 2024Article
- Vibrational Spectroscopy of Phytochromes.Biomolecules · 2023Review
- Ultrafast protein response in the Pfr state of Cph1 phytochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Long-Distance Protonation-Conformation Coupling in Phytochrome Species.Molecules (Basel, Switzerland) · 2022Article
- Protein control of photochemistry and transient intermediates in phytochromes.Nature communications · 2022Article
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological function of phytochromes is triggered by an ultrafast photoisomerization of the tetrapyrrole chromophore biliverdin between two rings denoted C and D. The mechanism by which this process induces extended structural changes of the protein is unclear. Here we report ultrafast proton-coupled photoisomerization upon excitation of the parent state (Pfr) of bacteriophytochrome Agp2. Transient deprotonation of the chromophore's pyrrole ring D or ring C into a hydrogen-bonded water cluster, revealed by a broad continuum infrared band, is triggered by electronic excitation, coherent oscillations and the sudden electric-field change in the excited state. Subsequently, a dominant fraction of the excited population relaxes back to the Pfr state, while ~35% follows the forward reaction to the photoproduct. A combination of quantum mechanics/molecular mechanics calculations and ultrafast visible and infrared spectroscopies demonstrates how proton-coupled dynamics in the excited state of Pfr leads to a restructured hydrogen-bond environment of early Lumi-F, which is interpreted as a trigger for downstream protein structural changes.
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