ArticleScientific reports2022
Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Beyond Composition: Structure-Activity Relationships in Bioactive Deep Eutectic Systems.Pharmaceutics · 2026Review
- Evaluation of Deep Eutectic Solvents for Cryopreservation of the Fish PathogenMethods and protocols · 2026Article
- Incorporation of a Natural Deep Eutectic Solvent-Based System as a Cryoprotectant in Solid Lipid Nanoparticles: Advancing toward Industrial Scalability.ACS nanoscience Au · 2025Article
- Deep Eutectic Systems: A Game Changer for Marine Bioactives Recovery.Marine drugs · 2025Review
- Optimization of Culture Medium for the Production of an Exopolysaccharide (p-CY02) with Cryoprotective Activity byJournal of microbiology and biotechnology · 2024Article
- Pharmaceutical applications of therapeutic deep eutectic systems (THEDES) in maximising drug delivery.Heliyon · 2024Review
- Predicting the formation of NADES using a transformer-based model.Scientific reports · 2024Article
- From Nature to Innovation: The Uncharted Potential of Natural Deep Eutectic Solvents.Molecules (Basel, Switzerland) · 2023Review
- Cryopreservation of tissues and organs: present, bottlenecks, and future.Frontiers in veterinary science · 2023Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this work we present the potential of Natural Deep Eutectic Systems (NADES) as new vitrification media for the cryopreservation of mammalian cells. Several NADES composed of natural metabolites were prepared and tested as CPAs in two cell lines, L929 and HacaT cells. After the harvesting, cells were mixed with the eutectic systems, and frozen directly into liquid nitrogen to achieve a vitreous state. Then, the cells were thawed and it was observed that NADES were able to exert a significant cryoprotective effect in L929 cells, when compared with DMSO or in the absence of a CPA. For HacaT cells, only a eutectic system showed a slightly improvement in cell survival, while DMSO caused complete cell death. Moreover, the thermal behaviour of the best systems was studied for further understanding the protective properties of NADES as CPAs, and have shown a significant difference in terms of T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.