ArticleNature communications2022
PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- High-throughput DNA engineering by mating bacteria.Cell systems · 2026Article
- Automated linear DNA assembly of A. thaliana's chloroplast and mitochondrial genome.Nucleic acids research · 2026Article
- Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.ACS synthetic biology · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- High-throughput yeast engineering in biofoundries: towards autonomous and scalable synthetic biology.FEMS yeast research · 2026Review
- UniClo technology exploits methylation for universal scarless DNA assembly.Biology methods & protocols · 2026Review
- CloneCoordinate: Open-Source Software for Collaborative DNA Construction.ACS synthetic biology · 2025Article
- On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology.Microbial biotechnology · 2025Review
- A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation.Communications biology · 2025Article
- A Mesophilic Argonaute fromBiomolecules · 2025Article
- PCR-Free Site-Directed Mutagenesis on Repetitive Sequences Using Single-Stranded DNA-Assisted Double-Stranded DNA Nicking by DNAzymes.Angewandte Chemie (International ed. in English) · 2025Article
- An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A generalized platform for artificial intelligence-powered autonomous enzyme engineering.Nature communications · 2025Article
- In vitro programmable DNA cleavage by a eukaryotic Argonaute.Nucleic acids research · 2025Article
- UniClo: scarless hierarchical DNA assembly without sequence constraint.Nucleic acids research · 2025Article
- Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder.Nature communications · 2025Article
- Self-resistance-gene-guided, high-throughput automated genome mining of bioactive natural products from Streptomyces.Cell systems · 2025Article
- Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.The Plant cell · 2025Article
- FAST-PB: An automated plant bioengineering system for scalable genome editing and phenotyping.The Plant cell · 2025Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in performing sophisticated biological experiments. Here, we report the development of a versatile, robust, automated end-to-end platform named PlasmidMaker that allows error-free construction of plasmids with virtually any sequences in a high throughput manner. This platform consists of a most versatile DNA assembly method using Pyrococcus furiosus Argonaute (PfAgo)-based artificial restriction enzymes, a user-friendly frontend for plasmid design, and a backend that streamlines the workflow and integration with a robotic system. As a proof of concept, we used this platform to generate 101 plasmids from six different species ranging from 5 to 18 kb in size from up to 11 DNA fragments. PlasmidMaker should greatly expand the potential of synthetic biology.
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