ArticleMethods in molecular biology (Clifton, N.J.)2022
Genotyping of African Swine Fever Virus.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 8 citations in OpenAlex.
- Chloroquine phosphate targets the MAPK-ERK pathway to inhibit ASFV SY-1 replication in vitro.Virology journal · 2026Article
- Article
- ANASFV: a workflow for African swine fever virus whole-genome analysis.Microbial genomics · 2025Article
- Transcriptome analysis of differences in the infection of African swine fever virus (SY-1 strain) in iPAMs and PAMs.Frontiers in immunology · 2025Article
- Modern experimental methods for assessing the effectiveness of tissue-engineered products for hyaline cartilage regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- African swine fever virus NAM P1/95 is a mixture of genotype I and genotype VIII viruses.Microbiology resource announcements · 2024Article
- Porcine respiratory disease complex: Dynamics of polymicrobial infections and management strategies after the introduction of the African swine fever.Frontiers in veterinary science · 2022Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral diseases of livestock. Real-time PCR (qPCR) is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, followed by Sanger sequencing and data analysis. Here, we describe the ASFV genotyping method used at the OIE Reference Laboratory for ASF at the Pirbright Institute, United Kingdom.
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