Evidence map›Paper›PMID 35575660›Full record

ArticleJBRA assisted reproduction2023

Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization.

Nilma De Souza Fernandes, Carla Grasiele Da Silva, Gean Pier Panizzon, Paula Motta Almodin Cerialle, Vânia Cibele Minguetti Câmara, Moacir Rafael Radaelli, Carlos Gilberto Almodin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JBRA assisted reproduction, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nilma De Souza FernandesMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.
Carla Grasiele Da SilvaMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.
Gean Pier PanizzonIngá Materiais Médico Hospitalares LTDA Maringá Brazil Ingá Materiais Médico Hospitalares LTDA, Maringá, Brazil.
Paula Motta Almodin CerialleMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.
Vânia Cibele Minguetti CâmaraMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.
Moacir Rafael RadaelliMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.
Carlos Gilberto AlmodinMaterbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo compare the efficacy of two density gradient centrifugation media for retrieving spermatozoa from semen samples by evaluating the total motile sperm count (TMSC) and the percentage recovery.

methodsTwenty-two men with different sperm counts participated in the study. The samples were divided into two equal aliquots and processed using the commercial ISolate Sperm Separation Medium (Irvine Scientific, United States) and the GV Gradiente (IngáMed, Brazil). After separation, samples were counted and evaluated for motile sperm recovery.

resultsThe mean TMSC in the fresh sample was 19.65±21.08 million/mL. After the ISolate separation the TMSC was 6.71±7.29 million/mL, and for the GV Gradiente it was 6.27±6.82 million/mL. The percentage of motile spermatozoa recovered was 36.47%±21.61 for ISolate and 35.22%±21.24 for the GV Gradiente (p>0.05). The samples from 6 oligospermic patients (27%) were evaluated separately and the TMSC for ISolate was 4.83±2.92 million/mL, and for the GV Gradiente, it was 4.16±3.12 million/mL (p=0.54). When evaluating only normospermic patient samples, the TMSC for ISolate was 9.05±7.29 million/mL, and for the GV Gradiente, it was 8.47±6.79 million/mL (p=0.83).

conclusionsThere was no statistical difference in retrieving motile sperm using the GV Gradiente and the ISolate Separation Medium.

Indexed as

SemenSperm MotilityCell SeparationCentrifugation, Density GradientFertilization in VitroHumansMaleSpermatozoaSperm CountDensity gradientfertilizationspermatozoa

Identifiers

PMID35575660
PMCPMC10065782

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.