Evidence map›Paper›PMID 35575079›Full record

ArticleThe Journal of heredity2022

Reference Genome Assembly of the Big Berry Manzanita (Arctostaphylos glauca).

Yi Huang, Merly Escalona, Glen Morrison, Mohan P A Marimuthu, Oanh Nguyen, Erin Toffelmier, H Bradley Shaffer, Amy Litt

Abstract read
In one paragraph

Article in The Journal of heredity, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Plastid Genome Assembly Using Long-read data.Molecular ecology resources · 2023
    Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yi HuangDepartment of Botany and Plant Science, University of California, Riverside, Riverside, CA 92521, USA.
Merly EscalonaDepartment of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Glen MorrisonDepartment of Botany and Plant Science, University of California, Riverside, Riverside, CA 92521, USA.
Mohan P A MarimuthuUC Davis Genome Center, DNA Technologies and Expression Analysis Cores, University of California, Davis, CA 95691, USA.
Oanh NguyenUC Davis Genome Center, DNA Technologies and Expression Analysis Cores, University of California, Davis, CA 95691, USA.
Erin ToffelmierDepartment of Ecology & Evolutionary Biology, University of California, Los Angeles, Los Angeles, CA 90095-7239, USA.
H Bradley ShafferDepartment of Ecology & Evolutionary Biology, University of California, Los Angeles, Los Angeles, CA 90095-7239, USA.
Amy LittDepartment of Botany and Plant Science, University of California, Riverside, Riverside, CA 92521, USA.ORCID 0000-0001-6709-8696

Funding

Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencinS10OD010786 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COMAI, LUCA · 2012 to 2012
$311k
NIH HHS S10 OD010786University of California, Riverside
6 · The paper itself

Abstract

Arctostaphylos (Ericaceae) species, commonly known as manzanitas, are an invaluable fire-adapted chaparral clade in the California Floristic Province (CFP), a world biodiversity hotspot on the west coast of North America. This diverse woody genus includes many rare and/or endangered taxa, and the genus plays essential ecological roles in native ecosystems. Despite their importance in conservation management, and the many ecological and evolutionary studies that have focused on manzanitas, virtually no research has been conducted on the genomics of any manzanita species. Here, we report the first genome assembly of a manzanita species, the widespread Arctostaphylos glauca. Consistent with the genomics strategy of the California Conservation Genomics project, we used Pacific Biosciences HiFi long reads and Hi-C chromatin-proximity sequencing technology to produce a de novo assembled genome. The assembly comprises a total of 271 scaffolds spanning 547Mb, close to the genome size estimated by flow cytometry. This assembly, with a scaffold N50 of 31Mb and BUSCO complete score of 98.2%, will be used as a reference genome for understanding the genetic diversity and the basis of adaptations of both common and rare and endangered manzanita species.

Indexed as

ArctostaphylosAnimalsEcosystemEndangered SpeciesFruitGenome SizeGenomicsCalifornia Conservation Genomics ProjectCalifornia Floristic ProvinceCCGPchaparralconservationendemic

Identifiers

PMID35575079
PMCPMC9113465

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.