Evidence map›Paper›PMID 35573685›Full record

ArticleFrontiers in cell and developmental biology2022

An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies.

Blaž Burja, Dominique Paul, Aizhan Tastanova, Sam G Edalat, Reto Gerber, Miranda Houtman, Muriel Elhai, Kristina Bürki, Ramon Staeger, Gaetana Restivo and 9 more

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In one paragraph

Article in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Blaž BurjaCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Dominique PaulDepartment of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
Aizhan TastanovaDepartment of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, Switzerland.
Sam G EdalatCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Reto GerberCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Miranda HoutmanCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Muriel ElhaiCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Kristina BürkiCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Ramon StaegerDepartment of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, Switzerland.
Gaetana RestivoDepartment of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, Switzerland.
Ramon LangDepartment of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, Switzerland.
Snezna Sodin-SemrlDepartment of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Katja LakotaDepartment of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Matija TomšičDepartment of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Mitchell P LevesqueDepartment of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, Switzerland.
Oliver DistlerCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Žiga RotarDepartment of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Mark D RobinsonDepartment of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
Mojca Frank-BertonceljCenter of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin. Our protocol enabled the isolation of a consistently high number of highly viable skin cells from small freshly dissociated punch skin biopsies, which we use for scRNA-seq studies. We recapitulated not only the main cell populations of existing single-cell skin atlases, but also identified rare cell populations, such as mast cells. Furthermore, we effectively isolated highly viable single cells from

Indexed as

ex vivo explantsprotocolscRNAseqskin biopsyskin tissue

Identifiers

PMID35573685
PMCPMC9096112

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.