ArticleClinical and experimental dental research2022
Cytotoxicity of 3D-printed, milled, and conventional oral splint resins to L929 cells and human gingival fibroblasts.
Article in Clinical and experimental dental research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Comparative Efficacy of Digital 3D-Printed and Conventional Stable Occlusal Splints in the Treatment of Temporomandibular Disorders.Journal of oral rehabilitation · 2025Trial
- In Vitro Cytotoxicity of Three Dimethacrylate-Based Photopolymer Resins for 3D-Printed Dental Restorations: A Qualitative Morphological Screening on Human Fibroblasts.Journal of functional biomaterials · 2026Article
- MTA does not induce oxidative stress and apoptotic response, or calcium-related cellular stress and preserves extracellular matrix homeostasis.Molecular biology reports · 2026Article
- Towards standardizing cytotoxicity testing of 3D-printed orthodontic aligners and retainers: a scoping review.Progress in orthodontics · 2026Article
- Biocompatibility and Cell Death Mechanisms Induced by PMMA-Based Dental Materials in Gingival Fibroblasts and OECM-1 Tumor Cells.Dentistry journal · 2026Article
- Evaluation of Post-Processing Time's Influence on Biocompatibility of 3D-Printed Denture Base Resins.Journal of functional biomaterials · 2026Article
- Flexural Strength, Monomer Release, and Wear of Occlusal Splint Materials Fabricated Through Conventional, Milled, or 3D-Printed Methods.Clinical and experimental dental research · 2026Article
- Transparent Transfer-Free Ultrasmall Multilayer Graphene Microelectrodes Enable High Quality Recordings in Brain Slices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Allergenic and cytotoxic potential of (meth)acrylate monomers in dental materials: a narrative review.Frontiers in dental medicine · 2026Review
- Three-Dimensionally Printed Splints in Dentistry: A Comprehensive Review.Dentistry journal · 2025Review
- Article
- Review
- Analysis of Gingival Fibroblasts Behaviour in the Presence of 3D-Printed versus Milled Methacrylate-Based Dental Resins-Do We Have a Winner?Journal of functional biomaterials · 2024Article
- Digital manufacturing techniques and the in vitro biocompatibility of acrylic-based occlusal device materials.Clinical oral investigations · 2024Article
- Cellular responses to 3D printed dental resins produced using a manufacturer recommended printer versus a third party printer.The journal of advanced prosthodontics · 2024Article
- Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances.Frontiers in bioengineering and biotechnology · 2024Article
- Disparity in the Influence of Implant Provisional Materials on Human Gingival Fibroblasts with Different Phases of Cell Settlement: An In Vitro Study.International journal of molecular sciences · 2023Article
- Design of Liposome Formulations for CRISPR/Cas9 Enzyme Immobilization: Evaluation of 5-Alpha-Reductase Enzyme Knockout for Androgenic Disorders.ACS omega · 2023Article
- Therapy for Temporomandibular Disorders: 3D-Printed Splints from Planning to Evaluation.Dentistry journal · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesEvidence on the biocompatibility of three-dimensional (3D)-printed and milled resins for oral splints is limited. This in vitro study assessed the influence of the manufacturing method on the cytotoxicity of oral splint resins on L929 cells and human gingival fibroblasts (GF1). MATERIALS AND
methodsStandardized specimens of four 3D-printed, two-milled, one-thermoformed, and one-pressed splint resin were incubated with L929 and GF1 cells for 24 h. Immunofluorescence and WST-8 assay were performed to evaluate cytotoxic effects. One-way analysis of variance and Tukey's multiple comparison test were applied with the variables "splint resin" and "manufacturing method" (p < .05).
resultsImmunofluorescence showed attachment of L929 and GF1 cells to the splint resins. Irrespective of the manufacturing method, the WST-8 assay revealed significant differences between splint resins for the viability of L929 and GF1 cells. L929 cells generally showed lower viability rates than GF1 cells. The evaluation of cell viability by the manufacturing method showed no significant differences between 3D printing, milling, and conventional methods.
conclusionsThe cytotoxic effects of 3D-printed, milled, and conventional oral splint resins were similar, indicating minor influence of the manufacturing method on biocompatibility. Cytotoxicity of the resins was below a critical threshold in GF1 cells. The chemical composition might be more crucial than the manufacturing method for the biocompatibility of splint resins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.