ArticleAMB Express2022
Transcriptomic analysis of Stropharia rugosoannulata reveals carbohydrate metabolism and cold resistance mechanisms under low-temperature stress.
Article in AMB Express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
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- A Review of Genomic, Transcriptomic, and Proteomic Applications in Edible Fungi Biology: Current Status and Future Directions.Journal of fungi (Basel, Switzerland) · 2025Review
- Genomic Insights intoJournal of fungi (Basel, Switzerland) · 2025Article
- Nutritional and Therapeutic Potential ofJournal of fungi (Basel, Switzerland) · 2025Review
- Analysis of Gene Regulatory Network and Transcription Factors in Different Tissues of theJournal of fungi (Basel, Switzerland) · 2025Article
- Identification of stable reference genes for qRT-PCR in Stropharia rugosoannulata using mRNA-sequencing data.PloS one · 2025Article
- Article
- Comparative Transcriptome Profiles of the Response of Mycelia of the GenusJournal of fungi (Basel, Switzerland) · 2024Article
- Integrated analysis of transcriptomics and metabolomics of peach under cold stress.Frontiers in plant science · 2023Article
- Correction: Transcriptomic analysis of Stropharia rugosoannulata reveals carbohydrate metabolism and cold resistance mechanisms under low-temperature stress.AMB Express · 2022Article
- Genomic Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Low temperature is an important environmental factor that restricts the growth of Stropharia rugosoannulata; however, the molecular mechanisms underlying S. rugosoannulata responses to low-temperature stress are largely unknown. In this study, we performed a transcriptome analysis of a high-sensitivity strain (DQ-1) and low-sensitivity strain (DQ-3) under low-temperature stress. The liquid hyphae of S. rugosoannulata treated at 25 °C and 10 °C were analyzed by RNA-Seq, and a total of 9499 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses showed that these genes were enriched in "xenobiotic biodegradation and metabolism", "carbohydrate metabolism", "lipid metabolism" and "oxidoreductase activity". Further research found that carbohydrate enzyme (AA, GH, CE, and GT) genes were downregulated more significantly in DQ-1 than DQ-3 and several cellulase activities were also reduced to a greater extent. Moreover, the CAT1, CAT2, GR, and POD genes and more heat shock protein genes (HSP20, HSP78 and sHSP) were upregulated in the two strains after low-temperature stress, and the GPX gene and more heat shock protein genes were upregulated in DQ-3. In addition, the enzyme activity and qRT-PCR results showed trends similar to those of the RNA-Seq results. This result indicates that low-temperature stress reduces the expression of different AA, GH, CE, and GT enzyme genes and reduces the secretion of cellulase, thereby reducing the carbohydrate metabolism process and mycelial growth of S. rugosoannulata. Moreover, the expression levels of different types of antioxidant enzymes and heat shock proteins are also crucial for S. rugosoannulata to resist low-temperature stress. In short, this study will provide a basis for further research on important signaling pathways, gene functions and variety breeding of S. rugosoannulata related to low-temperature stress.
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