ReviewPlants (Basel, Switzerland)2022
An Overview of Cryo-Scanning Electron Microscopy Techniques for Plant Imaging.
Review in Plants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Imaging of biofilms using cryo-scanning electron microscopy.Essays in biochemistry · 2026Review
- Ambient high-spatial-resolution mass spectrometry imaging: recent advances in technologies and metabolomics studies.Trends in analytical chemistry : TRAC · 2026Article
- Enhancing the Antioxidant Properties of Carrot Wastes Through Lactic Acid Fermentation and Thermophysical Treatments.Foods (Basel, Switzerland) · 2026Article
- Simple and Rapid Detection of Salivary Sheaths atInsects · 2026Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Advances in MoSBiosensors · 2025Review
- GRANA: An AI-based tool for accelerating chloroplast grana nanomorphology analysis using hybrid intelligence.Plant physiology · 2025Article
- Assessing the Impact of Microwaves and Other Disruptive Pretreatments onFoods (Basel, Switzerland) · 2025Article
- Review
- Advanced imaging-enabled understanding of cell wall remodeling mechanisms mediating plant drought stress tolerance.Frontiers in plant science · 2025Review
- Cryo-SEM Investigation ofBio-protocol · 2024Article
- Nanoscale Surface Metal-Coating Method without Pretreatment for High-Magnification Biological Observation and Applications.Biomimetics (Basel, Switzerland) · 2024Article
- Advanced environmental scanning electron microscopy reveals natural surface nano-morphology of condensed mitotic chromosomes in their native state.Scientific reports · 2024Article
- Reproductive Capacity and Scanning Electron Microscopy (SEM) Analyses of the Micromorphological Surfaces of Three EndemicPlants (Basel, Switzerland) · 2023Article
- Plasma-Derived Fibrin Hydrogels Containing Graphene Oxide for Infections Treatment.ACS materials letters · 2023Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many research questions require the study of plant morphology, in particular cells and tissues, as close to their native context as possible and without physical deformations from some preparatory chemical reagents or sample drying. Cryo-scanning electron microscopy (cryoSEM) involves rapid freezing and maintenance of the sample at an ultra-low temperature for detailed surface imaging by a scanning electron beam. The data are useful for exploring tissue/cell morphogenesis, plus an additional cryofracture/cryoplaning/milling step gives information on air and water spaces as well as subcellular ultrastructure. This review gives an overview from sample preparation through to imaging and a detailed account of how this has been applied across diverse areas of plant research. Future directions and improvements to the technique are discussed.
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Registered trials
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