Evidence map›Paper›PMID 35563747›Full record

ArticleCells2022

Bronchoalveolar-Lavage-Derived Fibroblast Cell Line (B-LSDM7) as a New Protocol for Investigating the Mechanisms of Idiopathic Pulmonary Fibrosis.

Laura Bergantini, Miriana d'Alessandro, Sara Gangi, Dalila Cavallaro, Giuseppe Campiani, Stefania Butini, Claudia Landi, Luca Bini, Paolo Cameli, Elena Bargagli

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Laura BergantiniRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.ORCID 0000-0002-1118-3223
Miriana d'AlessandroRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.ORCID 0000-0002-2368-5722
Sara GangiRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.
Dalila CavallaroRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.ORCID 0000-0002-7498-1791
Giuseppe CampianiDepartment of Biotechnology, Chemistry and Pharmacy, DoE Department of Excellence 2018-2022, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.ORCID 0000-0001-5295-9529
Stefania ButiniDepartment of Biotechnology, Chemistry and Pharmacy, DoE Department of Excellence 2018-2022, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.ORCID 0000-0002-8471-0880
Claudia LandiDepartment of Life Sciences, University of Siena, 53100 Siena, Italy.ORCID 0000-0003-0410-8038
Luca BiniDepartment of Life Sciences, University of Siena, 53100 Siena, Italy.ORCID 0000-0001-8951-2106
Paolo CameliRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.ORCID 0000-0001-8639-2882
Elena BargagliRespiratory Disease and Lung Transplant Unit, Department of Medical Sciences, Surgery and Neuroscience, Siena University, 53100 Siena, Italy.ORCID 0000-0002-8351-3703
University of Siena · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of BAL to study ILDs has improved our understanding of IPF pathogenesis. BAL fluid is routinely collected and can be considered a clinical and research tool. The procedure is well tolerated and minimally invasive. No specific cell lines from BAL or immortalized cell lines from IPF patients are available commercially. A method to quickly isolate and characterize fibroblasts from BAL is an unmet research need. MATERIALS AND

methodsHere we describe a new protocol by which we isolated a cell line from IPF. The cell line was expanded in vitro and characterized phenotypically, morphologically and functionally.

resultsThis culture showed highly filamentous cells with an evident central nucleus. From the phenotypic point of view, this cell line displays fibroblast/myofibroblast-like features including expression of alpha-SMA, vimentin, collagen type-1 and fibronectin. The results showed high expression of ROS in these cells. Oxidative stress invariably promotes extracellular matrix expression in lung diseases directly or through over-production of pro-fibrotic growth factors.

conclusionsOur protocol makes it possible to obtain fibroblasts BAL that is a routine non-invasive method that offers the possibility of having a large sample of patients. Standardized culture methods are important for a reliable model for testing molecules and eventual novel development therapeutic targets.

Indexed as

Idiopathic Pulmonary FibrosisBronchoalveolar Lavage FluidCell LineFibroblastsHumansTherapeutic IrrigationBALfibroblastIPF

Identifiers

PMID35563747
PMCPMC9103910
OpenAlexW4224323345

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.