Evidence map›Paper›PMID 35563654›Full record

ArticleInternational journal of molecular sciences2022

Fluorescence Lifetime Phasor Analysis of the Decamer-Dimer Equilibrium of Human Peroxiredoxin 1.

Sebastián F Villar, Joaquín Dalla-Rizza, Matías N Möller, Gerardo Ferrer-Sueta, Leonel Malacrida, David M Jameson, Ana Denicola

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Sebastián F VillarLaboratorio de Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.ORCID 0000-0002-3528-5232
Joaquín Dalla-RizzaLaboratorio de Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.
Matías N MöllerLaboratorio de Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.
Gerardo Ferrer-SuetaLaboratorio de Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.ORCID 0000-0002-8663-8480
Leonel MalacridaAdvanced Bioimaging Unit, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay.
David M JamesonDepartment of Cell and Molecular Biology, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Ana DenicolaLaboratorio de Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.ORCID 0000-0002-5181-9708
Universidad de la República de Uruguay · UYUniversity of Hawaiʻi at Mānoa · US

Funding

Membrane Remodeling by Arc/Arg3.1R21MH119516 · NIMH · UNIVERSITY OF HAWAII AT MANOA · PI ALBANESI, JOSEPH P, JAMESON, DAVID MICHAEL · 2019 to 2020
$425k
NIH HHS R21-MH119516NIMH NIH HHS R21 MH119516
6 · The paper itself

Abstract

Protein self-assembly is a common feature in biology and is often required for a myriad of fundamental processes, such as enzyme activity, signal transduction, and transport of solutes across membranes, among others. There are several techniques to find and assess homo-oligomer formation in proteins. Naturally, all these methods have their limitations, meaning that at least two or more different approaches are needed to characterize a case study. Herein, we present a new method to study protein associations using intrinsic fluorescence lifetime with phasors. In this case, the method is applied to determine the equilibrium dissociation constant (K

Indexed as

PeroxidasePeroxiredoxinsCysteineFluorescenceHumansCysteinePeroxidasePeroxiredoxinsdissociation constantlifetime phasorsperoxiredoxin 1protein oligomerizationspectral phasorstryptophan fluorescence

Identifiers

PMID35563654
PMCPMC9100220
OpenAlexW4229453732

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.