ReviewInternational journal of molecular sciences2022
Molecular Methodologies for Improved Polymicrobial Sepsis Diagnosis.
Review in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Descriptive Epidemiology ofBioMed research international · 2025Pooled it
- A rapid, inexpensive diagnostic for bacterial pathogen and resistance detection in resource-limited settings.Science advances · 2026Article
- Lean and Green Hand Surgery: Evaluating the Sterility of Stored Corticosteroid Injection Preparations Using Next-Generation Sequencing.Hand (New York, N.Y.) · 2026Article
- Calling a Truce: Enterococcus faecalis as a mediator between E. coli and C. albicans during polymicrobial CAUTIs.Research square · 2026Article
- A comparison of routine blood culture methods and multiplex quantitative PCR for detecting pathogens in simulated polymicrobial blood cultures.Journal of medical microbiology · 2026Article
- RNA Sequencing of Sepsis Patients Informs Tests to Quickly Diagnose Pathogens and Resistance.Shock (Augusta, Ga.) · 2026Article
- Comparison of targeted next-generation sequencing and conventional tests for pathogen detection in community-acquired and severe community-acquired pneumonia: a retrospective cohort study.Frontiers in microbiology · 2026Article
- Clinical diagnostic value of targeted next generation sequencing for lower respiratory tract infection: a retrospective study.Frontiers in cellular and infection microbiology · 2026Article
- Prevalence and microbiological profile of septic complications following ECMO decannulation: a prospective single-center study.Scientific reports · 2025Article
- Bacterial cell-free DNA profiling reveals the co-elevation of multiple bacteria in newborn foals with suspected sepsis.iScience · 2025Article
- Dual RNA isolation from blood: an optimized protocol for host and bacterial RNA purification for dual RNA-sequencing analysis in whole blood sepsis samples.Microbial genomics · 2025Article
- Impact of metagenomics next-generation sequencing on etiological diagnosis and early outcomes in sepsis.Journal of translational medicine · 2025Article
- Performance of ddPCR-GNB for microbial diagnosis of suspected bloodstream infection due to the four most common gram-negative bacteria: a prospective, multicenter study.Microbiology spectrum · 2025Article
- A Web-Based Dynamic Nomogram for Early Diagnosis in Sepsis: Development and Validation with Real-World Clinical Utility.Infection and drug resistance · 2025Article
- Seaside to Bedside: Assembly in Research for Emerging Human Fungal Pathogen Candida auris.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Advancing quantitative PCR with color cycle multiplex amplification.Nucleic acids research · 2024Article
- Evaluation of T2 Magnetic Resonance (T2MRAntibiotics (Basel, Switzerland) · 2024Article
- Getting Up to Speed: Rapid Pathogen and Antimicrobial Resistance Diagnostics in Sepsis.Microorganisms · 2024Review
- The Role of Biomarkers in Diagnosis of Sepsis and Acute Kidney Injury.Biomedicines · 2024Review
- Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration.Analytica chimica acta · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymicrobial sepsis is associated with worse patient outcomes than monomicrobial sepsis. Routinely used culture-dependent microbiological diagnostic techniques have low sensitivity, often leading to missed identification of all causative organisms. To overcome these limitations, culture-independent methods incorporating advanced molecular technologies have recently been explored. However, contamination, assay inhibition and interference from host DNA are issues that must be addressed before these methods can be relied on for routine clinical use. While the host component of the complex sepsis host-pathogen interplay is well described, less is known about the pathogen's role, including pathogen-pathogen interactions in polymicrobial sepsis. This review highlights the clinical significance of polymicrobial sepsis and addresses how promising alternative molecular microbiology methods can be improved to detect polymicrobial infections. It also discusses how the application of shotgun metagenomics can be used to uncover pathogen/pathogen interactions in polymicrobial sepsis cases and their potential role in the clinical course of this condition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.