ArticleJournal of orthopaedic surgery and research2022
Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon-bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells.
Article in Journal of orthopaedic surgery and research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Optimizing mesenchymal stem cell therapy for tendon-bone healing: Multifaceted approaches and future directions.World journal of stem cells · 2025Review
- Stem cell approaches for tendon repair: delusion or insight.The Journal of physiology · 2025Review
- Application and optimization of bioengineering strategies in facilitating tendon-bone healing.Biomedical engineering online · 2025Review
- Identification of rare genetic variants for rotator cuff tearing and repair in high-risk pedigrees.JSES international · 2024Article
- Human umbilical cord mesenchymal stem cells overexpressing RUNX1 promote tendon-bone healing by inhibiting osteolysis, enhancing osteogenesis and promoting angiogenesis.Genes & genomics · 2024Article
- Activation of CGRP receptor-mediated signaling promotes tendon-bone healing.Science advances · 2024Article
- Mesenchymal stem cells: An efficient cell therapy for tendon repair (Review).International journal of molecular medicine · 2023Review
- Mesenchymal stem cells and macrophages and their interactions in tendon-bone healing.Journal of orthopaedic translation · 2023Review
- Strategies to promote tendon-bone healing after anterior cruciate ligament reconstruction: Present and future.Frontiers in bioengineering and biotechnology · 2023Review
- Strategies for promoting tendon-bone healing: Current status and prospects.Frontiers in bioengineering and biotechnology · 2023Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAnterior cruciate ligament (ACL) injury could lead to functional impairment along with disabilities. ACL reconstruction often fails owing to the regeneration failure of tendon-bone interface. Herein, we aimed to investigate the effects of Runt related transcription factor 1 (RUNX1) on tendon-bone healing after ACL reconstruction using bone mesenchymal stem cells (BMSCs).
methodsBMSCs were isolated from the marrow cavity of rat femur, followed by the modification of RUNX1 with lentiviral system. Then, an ACL reconstruction model of rats was established with autografts.
resultsResults of flow cytometry exhibited positive-antigen CD44 and CD90, as well as negative-antigen CD34 and CD45 of the BMSCs. Then, we found that RUNX1-upregulated BMSCs elevated the decreased biomechanical strength of the tendon grafts after ACL reconstruction. Moreover, based on the histological observation, upregulation of RUNX1 was linked with better recovery around the bone tunnel, a tighter tendon-bone interface, and more collagen fibers compared to the group of BMSCs infected with LV-NC. Next, RUNX1-upregulated BMSCs promoted osteogenesis after ACL reconstruction, as evidenced by the mitigation of severe loss and erosion of the cartilage and bone in the tibial and femur area, as well as the increased number of osteoblasts identified by the upregulation of alkaline phosphatase, osteocalcin, and osteopontin in the tendon-bone interface.
conclusionElevated expression of RUNX1 contributed to tendon-bone healing after ACL reconstruction using BMSCs.
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