ArticleStem cell reports2022
Characterization of the epidermal-dermal junction in hiPSC-derived skin organoids.
Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 39 citations in OpenAlex.
- Skin Organoids in Proteostasis Research: Early Insights into Aging.Biomolecules & therapeutics · 2026Review
- Innovations in skin microphysiological systems for nonclinical testing and FDA modernization.Microsystems & nanoengineering · 2026Review
- Hair follicle organoids: advances in construction, applications, and translational challenges.Frontiers in cell and developmental biology · 2026Review
- Combining advanced 3D spheroid-based skin models with deep-learning-based image analysis enables in-depth investigation of keratinocyte differentiation and barrier function.Frontiers in bioengineering and biotechnology · 2026Article
- Review
- Human pluripotent stem cell-derived skin organoids enabled pathophysiological model of Mycobacterium tuberculosis infection.Nature communications · 2025Article
- Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem Cells.Advanced healthcare materials · 2025Article
- Lifting regenerative barriers promotes epithelial cell fate plasticity supporting lineage conversion.Nature communications · 2025Article
- Synergizing biomaterials and cellular molecular pathways: a new frontier in hair follicle generation.Journal of translational medicine · 2025Review
- Evaluation of canine epidermis and keratinocytoids (keratinocytic organoids) by transmission electron microscopy.Veterinary dermatology · 2025Article
- Recent progress in the identification and in vitro culture of skin organoids.Regenerative therapy · 2025Review
- Hydrodynamically generated multilayer skin spheroids enable in vitro screening of biologically active ingredients and toxicity tests.Science advances · 2025Article
- Biomedical applications of organoids in genetic diseases.Medical review (2021) · 2025Review
- Bioengineering strategies for regeneration of skin integrity: A literature review.Regenerative therapy · 2025Review
- Functional regeneration strategies of hair follicles: advances and challenges.Stem cell research & therapy · 2025Review
- Biomaterial-assisted organoid technology for disease modeling and drug screening.Materials today. Bio · 2025Review
- Organoids in skin wound healing.Burns & trauma · 2025Review
- Advances in engineered organoid models of skin for biomedical research.Burns & trauma · 2025Review
- Hair Regeneration Methods Using Cells Derived from Human Hair Follicles and Challenges to Overcome.Cells · 2024Review
- Efficient Generation of Skin Organoids from Pluripotent Cells via Defined Extracellular Matrix Cues and Morphogen Gradients in a Spindle-Shaped Microfluidic Device.Advanced healthcare materials · 2024Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via type I hemidesmosomes and integrin β1-based adhesion complexes. Importantly, we demonstrated that EDJs in organoids are almost devoid of type VII collagen, a fibril that mediates anchorage of the epidermis to dermis. This should be considered when using skin organoids for EB modeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.