ArticleVeterinary medicine and science2022
Effects of riboflavin on boar sperm motility, sperm quality, enzyme activity and antioxidant status during cryopreservation.
Article in Veterinary medicine and science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Metabolomic profiling of goat seminal plasma: insights into sperm motility regulation.BMC veterinary research · 2026Article
- Riboflavin Increases Goat Sperm Motility via Enhancement of Mitochondrial β-Oxidation.Biology · 2025Article
- Article
- Effects of hesperidin and ascorbic acid combination on boar sperm quality during cryopreservation.Frontiers in veterinary science · 2025Article
- Exploring the role of riboflavin in swine well-being: a literature review.Porcine health management · 2024Review
- Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen.Antioxidants (Basel, Switzerland) · 2023Article
- Effect of Magnetized Freezing Extender on Membrane Damages, Motility, and Fertility of Boar Sperm Following Cryopreservation.Animals : an open access journal from MDPI · 2023Article
- Effects of riboflavin on boar sperm motility, sperm quality, enzyme activity and antioxidant status during cryopreservation.Veterinary medicine and science · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThis study was conducted to evaluate the effect of adding riboflavin to boar sperm freezing extender on the challenge of cryopreservation.
methodsDifferent concentrations (0, 5, 10, 15, 20 or 25 μM) of riboflavin were added to the freezing extender. Spermatozoa motility, membrane integrity, acrosomal integrity, mitochondrial membrane potential and enzyme activities were analysed once 10 min after thawing. Q-PCR was used to detect the mRNA expression of Caspase3, Bcl-2 and Bax.
resultsThe results showed that the addition of 10 μM riboflavin to boar sperm freezing extender significantly increased the frozen-thawed sperm progressive motility compared with the control group (p < 0.05). Activities of superoxide dismutase, glutathione peroxidase and catalase improved after adding riboflavin to the extender (p < 0.05). During freezing-thawing, the boar sperm mitochondrial membrane potential, acrosomal integrity, plasma membrane and DNA at 10 μM in the riboflavin group increased by 6.6%, 9.6%, 5.49% and 5.62% (p < 0.05), respectively, compared with the control group. The addition of 10 μM riboflavin to the extender significantly decreased the malondialdehyde (p < 0.05) content, whereas it increased the ATP content (p < 0.05) of boar sperm during freezing-thawing. Furthermore, the expression of Caspase-3 and Bax (p < 0.05) were significantly lower, whereas the expression of BCL-2 (p < 0.05) was greater than the control group when adding 10 μM riboflavin to the extender.
conclusionsRiboflavin showed cryoprotective capacity to the freezing extender used for boar sperm during the process of freezing-thawing, and the optimal concentration of riboflavin for the frozen extender was 10 μM.
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