ArticleProceedings of the National Academy of Sciences of the United States of America2022
Targeted base editing in the mitochondrial genome of
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Research progress in gene editing of Brassicaceae crops.Biodesign research · 2026Review
- Harnessing mitochondrial genome editing for crop improvement: principles and applications.aBIOTECH · 2026Review
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance.Plant cell reports · 2026Review
- Chloroplast- and mitochondrion-specific random C-to-T mutagenesis for forward genetics of organelle genomes.The Plant journal : for cell and molecular biology · 2026Article
- TALENs and Related Technologies for Editing Nuclear and Organellar Genomes in a Model Plant,Bio-protocol · 2026Article
- High-frequency biparental inheritance of plant mitochondria upon chilling stress and loss of a genome-degrading nuclease.Nature plants · 2026Article
- Functional relocation of the maize chloroplast atpB gene to the nucleus restores photosynthetic competence to a gene-edited non-photosynthetic mutant.Plant biotechnology journal · 2025Article
- Genome modification in plant mitochondria.Plant physiology · 2025Review
- Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato.Plant biotechnology journal · 2025Article
- Reverse genetics in the Arabidopsis chloroplast genome identifies rps16 as a transcribed pseudogene.The Plant journal : for cell and molecular biology · 2025Article
- Targeted introduction of premature stop codon in plant mitochondrial mRNA by a designer pentatricopeptide repeat protein with C-to-U editing function.The Plant journal : for cell and molecular biology · 2025Article
- Resistance to the herbicide metribuzin conferred to Arabidopsis thaliana by targeted base editing of the chloroplast genome.Plant biotechnology journal · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- TALE-based C-to-T base editor for multiple homologous genes with flexible precision.Plant biotechnology (Tokyo, Japan) · 2024Article
- Strand-preferred base editing of organellar and nuclear genomes using CyDENT.Nature biotechnology · 2024Article
- Genome Editing of Plant Mitochondrial and Chloroplast Genomes.Plant & cell physiology · 2024Review
- Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs.Plant biotechnology journal · 2024Article
- Targeted A-to-G base editing in the organellar genomes of Arabidopsis with monomeric programmable deaminases.Plant physiology · 2024Article
- TALE-based organellar genome editing and gene expression in plants.Plant cell reports · 2024Review
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beyond their well-known role in respiration, mitochondria of land plants contain biologically essential and/or agriculturally important genes whose function and regulation are not fully understood. Until recently, it has been difficult to analyze these genes or, in the case of crops, to improve their functions, due to a lack of methods for stably modifying plant mitochondrial genomes. In rice, rapeseed, and Arabidopsis thaliana, mitochondria-targeting transcription activator-like effector nucleases (mitoTALENs) have recently been used to disrupt targeted genes in an inheritable and stable manner. However, this technique can also induce large deletions around the targeted sites, as well as cause ectopic homologous recombinations, which can change the sequences and gene order of mitochondrial genomes. Here, we used mitochondria-targeting TALEN-based cytidine deaminase to successfully substitute targeted C:G pairs with T:A pairs in the mitochondrial genomes of plantlets of A. thaliana without causing deletions or changes in genome structure. Expression vectors of the base editor genes were stably introduced into the nuclear genome by the easy-to-use floral dipping method. Some T1 plants had apparent homoplasmic substitutions that were stably inherited by seed progenies, independently of the inheritance of nuclear-introduced genes. As a demonstration of the method, we used it to restore the growth of an organelle transcript processing 87 (otp87) mutant that is defective in the editing of RNA transcripts of the mitochondrial atp1 gene and to identify bases in atp1 that affect the efficiency of RNA editing by OTP87.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.