ArticlePlant biotechnology journal2022
CRISPR/Cas9-mediated tetra-allelic mutation of the 'Green Revolution' SEMIDWARF-1 (SD-1) gene confers lodging resistance in tef (Eragrostis tef).
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies forbioRxiv : the preprint server for biology · 2025Article
- Toward improving multiomic research in understudied cereals.Nature genetics · 2025Review
- Millets for a sustainable future.Journal of experimental botany · 2025Review
- Genetic diversity and environmental adaptation in Ethiopian tef.G3 (Bethesda, Md.) · 2025Article
- Application of machine learning and genomics for orphan crop improvement.Nature communications · 2025Review
- Exploring the phenotypic diversity ofFrontiers in plant science · 2025Article
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- From 'Farm to Fork': Exploring the Potential of Nutrient-Rich and Stress-Resilient Emergent Crops for Sustainable and Healthy Food in the Mediterranean Region in the Face of Climate Change Challenges.Plants (Basel, Switzerland) · 2024Review
- Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype.BMC plant biology · 2024Article
- Navigating biosafety regulatory frameworks for genetic engineering in Africa: a focus on genome editing and gene drive technologies.Frontiers in bioengineering and biotechnology · 2024Article
- Biggest of tinies: natural variation in seed size and mineral distribution in the ancient crop tef [Frontiers in plant science · 2024Article
- 3Bs of CRISPR-Cas mediated genome editing in plants: exploring the basics, bioinformatics and biosafety landscape.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- CRISPR-Cas System, a Possible "Savior" of Rice Threatened by Climate Change: An Updated Review.Rice (New York, N.Y.) · 2023Review
- Grain shattering by cell death and fracture in Eragrostis tef.Plant physiology · 2023Article
- Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum.Nature plants · 2023Article
- Multifaceted roles of transcription factors during plant embryogenesis.Frontiers in plant science · 2023Review
- Green revolution to genome revolution: driving better resilient crops against environmental instability.Frontiers in genetics · 2023Review
- CRISPR/Cas9-mediated tetra-allelic mutation of the 'Green Revolution' SEMIDWARF-1 (SD-1) gene confers lodging resistance in tef (Eragrostis tef).Plant biotechnology journal · 2022Article
- CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects.Frontiers in genetics · 2022Review
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19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tef is a staple food and a valuable cash crop for millions of people in Ethiopia. Lodging is a major limitation to tef production, and for decades, the development of lodging resistant varieties proved difficult with conventional breeding approaches. We used CRISPR/Cas9 to introduce knockout mutations in the tef orthologue of the rice SEMIDWARF-1 (SD-1) gene to confer semidwarfism and ultimately lodging resistance. High frequency recovery of transgenic and SD-1 edited tef lines was achieved in two tef cultivars by Agrobacterium-mediated delivery into young leaf explants of gene editing reagents along with transformation and regeneration enhancing morphogenic genes, BABY BOOM (BBM) and WUSCHEL2 (WUS2). All of the 23 lines analyzed by next-generation sequencing had at least two or more alleles of SD-1 mutated. Of these, 83% had tetra-allelic frameshift mutations in the SD-1 gene in primary tef regenerants, which were inherited in subsequent generations. Phenotypic data generated on T
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