Evidence map›Paper›PMID 35559592›Full record

ArticleAngewandte Chemie (International ed. in English)2022

Amplification-Free Detection of SARS-CoV-2 and Respiratory Syncytial Virus Using CRISPR Cas13a and Graphene Field-Effect Transistors.

Huijie Li, Jie Yang, Guangfu Wu, Zhengyan Weng, Yang Song, Yuxuan Zhang, Jeffrey A Vanegas, Lori Avery, Zan Gao, He Sun and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Huijie LiDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Jie YangDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Guangfu WuDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Zhengyan WengDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Yang SongDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Yuxuan ZhangDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Jeffrey A VanegasDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Lori AveryDepartment of Pathology and Laboratory Medicine, UConn Health, Farmington, CT 06030, USA.
Zan GaoDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
He SunDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Yupeng ChenDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0001-6940-6277
Kevin D DieckhausDivision of Infectious Diseases, Department of Medicine, UConn Health, Farmington, CT 06030, USA.
Xue GaoDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Yi ZhangDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0002-0907-663X

Funding

Supplement: Developing Nanomaterial Platform for Intra-Cartilage Delivery of RNA Therapeutics against Joint DiseasesR01AR072027 · NIAMS · UNIVERSITY OF CONNECTICUT STORRS · PI CHEN, YUPENG · 2017 to 2022
$2.3M
NIAMS NIH HHS R01 AR072027
6 · The paper itself

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors in infectious respiratory disease diagnostic applications still require target preamplifications. This study reports a new biosensor for amplification-free nucleic acid detection via harnessing the trans-cleavage mechanism of Cas13a and ultrasensitive graphene field-effect transistors (gFETs). CRISPR Cas13a-gFET achieves the detection of SARS-CoV-2 and respiratory syncytial virus (RSV) genome down to 1 attomolar without target preamplifications. Additionally, we validate the detection performance using clinical SARS-CoV-2 samples, including those with low viral loads (Ct value >30). Overall, these findings establish our CRISPR Cas13a-gFET among the most sensitive amplification-free nucleic acid diagnostic platforms to date.

Indexed as

COVID-19GraphiteNucleic AcidsCRISPR-Cas SystemsHumansRespiratory Syncytial VirusesSARS-CoV-2GraphiteNucleic AcidsAmplification-Free DetectionBiosensorsCRISPR Cas13aGraphene Field-Effect TransistorsSARS-CoV-2

Identifiers

PMID35559592
PMCPMC9347639

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.