ArticlePlant molecular biology2022
Molecular and biochemical characterization of two 4-coumarate: CoA ligase genes in tea plant (Camellia sinensis).
Article in Plant molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 38 citations in OpenAlex.
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- Metabolomics-based elucidation of the chemical basis under-lying seasonal variations in sensory quality of Goldsands Black Tea.Frontiers in plant science · 2026Article
- The Phenylpropanoid Gatekeeper CtPAL1 Coordinates ABA-Induced Flavonoid Biosynthesis and Oxidative Stress Tolerance in Safflower (Plants (Basel, Switzerland) · 2025Article
- Dual omics comparison: how Agrobacterium tumefaciens and Agrobacterium rhizogenes modulate gene expression and metabolism in Hypericum perforatum L.BMC genomics · 2025Article
- CoExpPhylo - a novel pipeline for biosynthesis gene discovery.BMC genomics · 2025Article
- Phosphoproteomic Analysis Reveals Impairment of Rice Germination by Chloramphenicol.Plants (Basel, Switzerland) · 2025Article
- Biosynthetic Mechanisms of Plant Chlorogenic Acid from a Microbiological Perspective.Microorganisms · 2025Review
- Integrating transcriptome and metabolome analyses to characterize flower development in Salix variegata franch., a typical dioecious plant.Scientific reports · 2025Article
- Integrative transcriptomics and metabolomics reveal the biosynthesis of flavonoid metabolites inFrontiers in plant science · 2025Article
- Identifying meteorological factors influencing catechin biosynthesis and optimizing cultivation conditions of tea plant (Frontiers in plant science · 2025Article
- Integrated Metabolomics and Transcriptomics Provide Key Molecular Insights into Floral Stage-Driven Flavonoid Pathway in Safflower.International journal of molecular sciences · 2024Article
- Chromosome-Scale Genome Assembly and Characterization of Top-Quality Japanese Green Tea Cultivar 'Seimei'.Plant & cell physiology · 2024Article
- 6-BA Reduced Yield Loss under Waterlogging Stress by Regulating the Phenylpropanoid Pathway in Wheat.Plants (Basel, Switzerland) · 2024Article
- Cloning and functional analysis of Gb4CL1 and Gb4CL2 from Ginkgo biloba.The plant genome · 2024Article
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- Analysis of Functional Single-Nucleotide Polymorphisms (SNPs) and Leaf Quality in Tea Collection under Nitrogen-Deficient Conditions.International journal of molecular sciences · 2023Article
- A Review of Classification, Biosynthesis, Biological Activities and Potential Applications of Flavonoids.Molecules (Basel, Switzerland) · 2023Review
- The Classification, Molecular Structure and Biological Biosynthesis of Flavonoids, and Their Roles in Biotic and Abiotic Stresses.Molecules (Basel, Switzerland) · 2023Review
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9 authors at 2 institutions in 2 countries.
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Abstract
key messageTwo 4-coumarate: CoA ligase genes in tea plant involved in phenylpropanoids biosynthesis and response to environmental stresses. Tea plant is rich in flavonoids benefiting human health. Lignin is essential for tea plant growth. Both flavonoids and lignin defend plants from stresses. The biosynthesis of lignin and flavonoids shares a key intermediate, 4-coumaroyl-CoA, which is formed from 4-coumaric acid catalyzed by 4-coumaric acid: CoA ligase (4CL). Herein, we report two 4CL paralogs from tea plant, Cs4CL1 and Cs4CL2, which are a member of class I and II of this gene family, respectively. Cs4CL1 was mainly expressed in roots and stems, while Cs4CL2 was mainly expressed in leaves. The promoter of Cs4CL1 had AC, nine types of light sensitive (LSE), four types of stress-inducible (SIE), and two types of meristem-specific elements (MSE). The promoter of Cs4CL2 also had AC and nine types of LSEs, but only had two types of SIEs and did not have MSEs. In addition, the LSEs varied in the two promoters. Based on the different features of regulatory elements, three stress treatments were tested to understand their expression responses to different conditions. The resulting data indicated that the expression of Cs4CL1 was sensitive to mechanical wounding, while the expression of Cs4CL2 was UV-B-inducible. Enzymatic assays showed that both recombinant Cs4CL1 and Cs4CL2 transformed 4-coumaric acid (CM), ferulic acid (FR), and caffeic acid (CF) to their corresponding CoA ethers. Kinetic analysis indicated that the recombinant Cs4CL1 preferred to catalyze CF, while the recombinant Cs4CL2 favored to catalyze CM. The overexpression of both Cs4CL1 and Cs4CL2 increased the levels of chlorogenic acid and total lignin in transgenic tobacco seedlings. In addition, the overexpression of Cs4CL2 consistently increased the levels of three flavonoid compounds. These findings indicate the differences of Cs4CL1 and Cs4CL2 in the phenylpropanoid metabolism.
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